INSULIN SENSITIVITY AND INTRACELLULAR SIGNALING OF MUSCLE PROTEIN SYNTHESIS IN

胰岛素敏感性和肌肉蛋白合成的细胞内信号转导

• 批准号: 7605403
• 负责人: ARNY A FERRANDO
• 金额: $ 0.11万
• 依托单位: UNIVERSITY OF TEXAS MED BR GALVESTON
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7605403
• 关键词: Age; Aging; Cells; Coin; Computer Retrieval of Information on Scientific Projects Database; Data; Defect; Development; Elderly; Exercise; Experimental Designs; Funding; Grant; Hour; Infusion procedures; Institution; Insulin; Insulin Resistance; Lead; Leg; Muscle; Muscle Proteins; Pilot Projects; Procedures; Protein Biosynthesis; Purpose; Research; Research Personnel; Resistance; Resources; Rest; Screening procedure; Signal Transduction; Source; Time; United States National Institutes of Health; Visit; femoral artery; improved; insulin sensitivity; muscle metabolism; response; sarcopenia

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Background: The development of insulin resistance is a common feature of aging. Loss of muscle mass and strength with aging is also an increasingly common problem. There is evidence that elderly muscle does not respond to insulin in the same manner as young muscle. The purpose of this study is to determine the response of muscle tissue to insulin and resistive exercise in the elderly. Hypothesis: We hypothesize that insulin resistance in the elderly contributes to loss of muscle mass. We also hypothesize that resistance exercise improves the loss of muscle in the elderly. Specific Aims and Procedures (summary): 1. To determine the factors associated with changes in muscle protein synthesis in the elderly in response to insulin and resistive exercise. 2. To determine the time course of the response of the muscle tissue to the resistive exercise, insulin and, the combination of insulin and resistive exercise. The data from this pilot study will be used in planning a larger trial investigating the relationship between insulin resistance and muscle loss in the elderly. Experimental Design (summary): This study will include normal healthy elderly between the ages of 60-85 who do not participate in any form of resistive exercise. Subjects will undergo a comprehensive screening visit prior to participating in the study. Subjects will be randomly selected (for example, by the flip of a coin), to participate in one of three studies. Study 1 will involve a period of rest followed by infusion of insulin for 6 hours into a femoral artery. Study 2 will involve a resistance exercise workout of the legs (i.e. four different leg exercises), followed by an 8-hour insulin infusion period. Study 3 will involve the same resistance exercise workout as Study 2 but will be followed by 8 hours of rest with no insulin infusion. Significance (summary): Identification of the cells responsible for defects in muscle metabolism that lead to muscle loss and aging will provide the information needed for development of effective therapies to treat sarcopenia.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 背景：胰岛素抵抗的发展是衰老的一个共同特征。随着年龄的增长，肌肉质量和力量的损失也是一个越来越普遍的问题。有证据表明，老年肌肉对胰岛素的反应与年轻肌肉不同。本研究的目的是确定老年人肌肉组织对胰岛素和抗阻运动的反应。 假设：我们假设老年人的胰岛素抵抗导致肌肉质量的损失。 我们还假设，抗阻运动改善了老年人的肌肉损失。 具体目标和程序（摘要）： 1.确定与老年人对胰岛素和抗阻运动反应的肌肉蛋白质合成变化相关的因素。 2.为了确定肌肉组织对抗阻运动、胰岛素以及胰岛素和抗阻运动的组合的反应的时间过程。 这项初步研究的数据将用于计划一项更大的试验，调查老年人胰岛素抵抗和肌肉损失之间的关系。 实验设计（总结）：这项研究将包括年龄在60-85岁之间的正常健康老年人，他们不参加任何形式的抗阻运动。受试者将在参加研究前接受全面筛选访视。将随机选择受试者（例如，通过抛硬币），参与三项研究之一。研究1将涉及休息一段时间，然后将胰岛素输注到股动脉中6小时。研究2将涉及腿部阻力运动锻炼（即四种不同的腿部运动），随后是8小时胰岛素输注期。研究3将涉及与研究2相同的抗阻运动锻炼，但随后将休息8小时，不输注胰岛素。 意义（总结）：鉴定导致肌肉损失和衰老的肌肉代谢缺陷的细胞将为开发治疗肌肉减少症的有效疗法提供所需的信息。