INFLUENZA IMMUNITY: PROTECTIVE MECHANISMS AGAINST A PRV

流感免疫：针对 PRV 的保护机制

• 批准号: 7605191
• 负责人: CORNELIA L DEKKER
• 金额: $ 0.23万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-02-15 至 2007-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7605191
• 关键词: Adult; Attenuated; B-Lymphocytes; Biological Assay; Biological Models; Bioterrorism; Blood specimen; CD4 Positive T Lymphocytes; CD8B1 gene; Characteristics; Child; Computer Retrieval of Information on Scientific Projects Database; Elderly; Flushield; Fluzone; Funding; Grant; Human; Immune; Immune response; Immunity; Immunization; Influenza; Institution; Intramuscular Injections; Learning; Licensing; Life; Modeling; Natural Immunity; Natural Killer Cells; Population; Randomized; Readiness; Research; Research Personnel; Resources; Respiratory System; Source; T-Lymphocyte; Time; United States National Institutes of Health; Vaccines; antimicrobial; biodefense; immunogenicity; influenza virus vaccine; microbial; pandemic disease; response

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The fundamental objective of our Stanford Cooperative Center is to use the analysis of vaccine-induced and naturally-acquired influenza A immunity as a model for defining adaptive and innate immune mechanisms and antimicrobial protection of the respiratory tract in children and younger and older adults. Influenza A was chosen as a model system relevant to biodefense because influenza A has the potential to be modified or manipulated genetically to produce a microbial agent of bioterrorism. Furthermore, influenza A causes natural pandemics, which incapacitate a large fraction of the adult population within a short time-frame, and may endanger defense preparedness. In either circumstance, influenza A has many characteristics of microbial agents that could become civilian bioterrorist agents. Our scientific objective is to study the human host response, with the focus on the humoral and cellular immune responses of both children and adults to vaccine-induced influenza A stimulation. We hope to learn how the immune responses to either live, attenuated influenza vaccine or inactivated influenza vaccine compares between adults and children by studying CD4 T-cells, CD8 T-cells, B-cell immunity and natural killer (NK) cell responses to influenza A before and after immunization. In yr 1, we propose to immunize 64 children 5-9 yrs old and 64 adults 19-49 yrs old. They will be randomized to receive one of two licensed products with a 1:1 allocation: either FluMist (live, attenuated flu vaccine) via intranasal spray or Fluzone (inactivated influenza vaccine) via intramuscular (IM) injection. Blood samples for immunogenicity studies will be drawn prior to immunization and one-month post-immunization for immunogenicity assays.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们的斯坦福大学合作中心的基本目标是使用疫苗诱导和自然获得的甲型流感免疫的分析作为模型，用于定义儿童和年轻人和老年人的呼吸道的适应性和先天性免疫机制和抗菌保护。 选择甲型流感作为与生物防御有关的模型系统，因为甲型流感有可能被基因修饰或操纵，以产生生物恐怖主义的微生物剂。 此外，甲型流感引起自然大流行，在短时间内使大部分成年人丧失能力，并可能危及防御准备。 在任何一种情况下，甲型流感都具有微生物制剂的许多特征，可能成为民用生物恐怖制剂。 我们的科学目标是研究人类宿主反应，重点是儿童和成人对疫苗诱导的甲型流感刺激的体液和细胞免疫反应。 我们希望通过研究免疫接种前后对甲型流感的CD 4 T细胞、CD 8 T细胞、B细胞免疫和自然杀伤（NK）细胞反应，了解成人和儿童对减毒活流感疫苗或灭活流感疫苗的免疫反应。 在第一年，我们建议免疫64名5-9岁的儿童和64名19-49岁的成年人。 他们将被随机分配接受两种许可产品之一，分配比例为1：1：通过鼻内喷雾的FluMist（减毒活流感疫苗）或通过肌内（IM）注射的Fluzone（灭活流感疫苗）。 将在免疫前和免疫后1个月采集用于免疫原性研究的血样，用于免疫原性试验。