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Microfluidic Temperature Steps to Understand Robustness of Embryonic Development

了解胚胎发育稳健性的微流体温度步骤

基本信息

批准号：
7595777
负责人：
RUSTEM F ISMAGILOV
金额：
$ 17.44万
依托单位：
UNIVERSITY OF CHICAGO
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-04-01 至 2010-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7595777
关键词：
Affect Aging Animal Model Area Biochemical Biochemical Process Biological Cells Collaborations Communities Confocal Microscopy Coupled Coupling Defect Development Developmental Biology Developmental Process Drosophila genus Drosophila melanogaster Embryo Embryonic Development Ensure Environment Equipment Genetic Genomics Human Development Image Individual Laboratories Life Liquid substance Malignant Neoplasms Methods Microfluidic Microchips Microfluidics Microscopy Modeling Molecular Molecular Biology Movement Nuclear Operative Surgical Procedures Organism Pathway interactions Pattern Photons Process Proteins Proteomics Research Research Personnel Resolution Stream Surface Techniques Technology Temperature Testing Time Work base biological systems design fluid flow human disease improved molecular dynamics nuclear division professor programs simulation time use tool two-photon warm temperature

项目摘要

Project summary. This proposal describes a multi-disciplinary research program that aims to develop, validate and disseminate microfluidic technology to allow development of a live Drosophila embryo to be controlled in space and time using temperature steps. The process of Drosophila embryo development is robust - it works precisely even under varying environmental conditions such as temperature. While the function of many individual molecules present in Drosophila development is known, it is unknown how these molecules work together to make developmental network robust. New microfluidic technology that can differentially control temperature around different parts a living embryo could become a powerful tool in determining the mechanisms responsible for this robustness of development. Specific Aim 1focuses on development of new microfluidic technology that will use laminar flow to create a sharp temperature step around the embryo, where one part of the embryo will develop at the warmer temperature of one laminar stream, and the other part of the embryo will develop at the cooler temperature of the second laminar stream. The temperature profile at the surface of the embryo will be quantitatively characterized using numerical simulations and confocal microscopy. Real-time imaging of an embryo being exposed to temperature step is critical in identifying dynamic processes such as changes in protein concentration as a function of time. Specific Aim 2 adapts technology developed in Specific Aim 1to DIG and 2-photon microscopy in order to image embryonic development in the temperature step in real time. Proposed research in Specific Aim 3 will validate the microfluidic technology developed in Specific Aim 1and Specific Aim 2 by answering four important questions concerning the mechanism of robustness, both at the molecular level and at the level of nuclear divisions. Relevance: Understanding development is essential for understanding of human diseases and conditions caused by defects and errors in developmental and differentiation pathways (such as cancer and aging). Studying development in model organisms¿in particular, the fruit fly Drosophila melanogaster¿leads to a better understanding of human development, since many parts of the basic machinery of development are similar among organisms. The microfluidic technology developed in this proposal will enable understanding of the mechanism that provides error-free operation of developmental network in the fruit fly Drosophila melanogaster, and this technology will be extendable to testing and understanding errors in development of other model organisms

项目总结。该提案描述了一个多学科研究项目，旨在发展，