WILLIAM WEIS PRT TIME: ADHESION MOLECULE AND HIV

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 该计划旨在确定参与细胞膜靶向、黏附和信号传递过程的蛋白质的结构，以建立它们相互作用的机制和特异性。有三个主要领域正在研究中，所有这些领域都需要同步辐射的晶体。1.免疫系统中细胞表面碳水化合物的识别，重点是血清甘露糖结合蛋白及其如何触发补体介导的细胞裂解，以及DC-SIGN，它是一种黏附分子，介导抗原呈递细胞激活T细胞，也用于捕获艾滋病毒并将其输送到T细胞。2.细胞间连接的构建和组装，研究连接钙粘附素细胞黏附分子和细胞骨架的蛋白质之间的相互作用。粘着连接蛋白β-catenin也是Wnt信号通路的重要组成部分，它与其他Wnt通路成分的相互作用也在研究中。3.负责胞内囊泡与靶膜的特异性对接和融合的蛋白质。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This program aims to determine the structures of proteins involved in cell membrane targeting, adhesion and signaling processes, in order to establish the mechanism and specificity of their interactions. There are three principal areas under investigation, all of which with crystals that require synchrotron radiation. 1. Cell-surface carbohydrate recognition in the immune system, focusing on serum Mannose-binding Proteins and how they trigger complement-mediated cell lysis, and DC-SIGN, an adhesion molecule that mediates activation of T cells by antigen-presenting cells, and which also serves to capture HIV and deliver it to T cells. 2. Architecture and assembly of intercellular junctions, studying the interactions of the proteins that link cadherin cell adhesion molecules to the cytoskeleton. The adherens junction protein beta-catenin is also an essential component of the Wnt signaling pathway, and its interactions with other Wnt pathway components are also being studied. 3. Proteins responsible for specific docking and fusion of intracellular vesicles with target membranes.