SIGNAL TRANSDUCTION COMPLEXES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 这个项目涉及两个蛋白质，光转导的异三聚体G蛋白的α亚基转导蛋白(Gat)及其效应酶cGMP磷酸二酯酶(PDE6)，一个具有两个催化亚基(Ab)和两个抑制亚基(Rr)的异四聚体蛋白。PDE6和Gat都是具有共价结合脂质的外周膜蛋白，我们研究了它们在溶液(冰)中的络合物和附着在脂质双层膜上的络合物。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project involves two proteins, the alpha subunit of the heterotrimeric G protein of phototransduction, transducin (Gat), and its effector enzyme, cGMP phosphodiesterase (PDE6), a heterotetrameric protein with two catalytic (ab) and two inhibitory subunits (rr). Both PDE6 and Gat are peripheral membrane proteins with covalently attached lipids and we have studied their complexes both in solution (ice) and attached to lipid bilayer membranes.