PARTICLE TRACKING WITH MPM/OCT

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 之前，我们团队使用2D相关分析对锚定蛋白Paxlin的作用进行了研究，这种锚定蛋白对细胞黏附的形成是必不可少的。我们的模型组织由嵌入在3D胶原基质凝胶中的细胞组成，其中胶原基质用于模拟ECM。使用多光子荧光(MPM)、二次谐波(SHG)和光学相干层析(OCT)的多模式系统，我们能够成像细胞与ECM的相互作用，如细胞的运动和在ECM中产生的变形。我们能够使用时空图像相关光谱(STICS)从OCT和MPM通道获得的信号来确定图像中每个点的位移和速度。此外，我们还想研究Paxlin等粘着斑蛋白的积聚与细胞运动之间的关系。最终，当我们测量ECM中的变形时，我们想要提取施加在各种细胞粘连上的力。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Previous studies using correlation analyses in 2D have been performed by our group about the role of Paxilin, an anchor protein, that is essential for the formation of cellular adhesions. Our model tissue was comprised of cells embedded in a 3D collagen matrix gel where the collagen matrix is used to simulate the ECM. Using the multi-modal system of multi-photon fluorescence (MPM), second harmonic generation (SHG) and optical coherence tomography (OCT), we are able to image the interactions of the cell with the ECM such as the movement of the cell and the resulting deformation in the ECM. We were able to determine the displacement and the velocities at every point in the image using Spatio-Temporal Image Correlation Spectroscopy STICS) from the signals obtained from the OCT and MPM channels. Furthermore we would like to study the relationship between the accumulation of the focal adhesion proteins such as Paxilin and the movement of the cell. Ultimately, as we measure the deformation in the ECM, we would like to extract the force applied at the various cell adhesions.