ACTIVE STAINING FOR DEVELOPMENTAL TOXICOLOGY

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 本项目的目的是建立18-21日龄大鼠胚胎和21日龄仔鼠的主动染色和扫描方法，为今后发育生物学和发育毒理学的研究提供必要的基础。 更具体地说，我们将做以下工作： -开发将Gd造影剂(ProHance)携带到标本中以降低T1的灌流方法 -使用定量T1/T2直方图滴定浓度，以确定松弛时间在选定的冠状切片中的分布；开发一种3D采集协议，允许在每次扫描8小时内获得尽可能高的SNR和器官清晰度 -确定18-21天大鼠胚胎常规磁共振组织学所需的分辨率；确定21天小鼠常规磁共振组织学所需的分辨率 -了解如何为MR组织学制定具有生物学意义的SOP。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of this project is to develop methods for active staining and scanning of 18-21 day rat fetuses and 21 day old rat pups to provide the necessary foundation for future studies in developmental biology and developmental toxicology. More specifically we will do the following: --- develop perfusion methods for carrying Gd contrast agent (ProHance) into the specimen to reduce T1 --- titrate the concentration using quantitative T1/T2 histograms to determine the distribution of relaxation times in selected coronal slices; develop a 3D acquisition protocol that will allow the highest possible SNR, and organ definition in 8 hours per scan --- determine the resolution required for routine MR histology in the 18-21 day rat fetus; determine the resolution required for routine MR histology of the 21 day rat pup --- understand how to develop a biologically relevant SOP for MR histology.