MEASUREMENT OF ANALYTES IN WHOLE BLOOD AND SERUM

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 在对患者的治疗方案进行评估时，快速可靠地测量与临床相关的分析物水平对于医生来说是非常宝贵的。目前的临床实验室检测涉及样品的准备、试剂的使用，而且需要大量的时间。拉曼光谱很有吸引力，因为它可以同时测量多个分析物，具有很高的特异性。在过去的研究中，近红外拉曼已被证明能够检测低毫米级范围内的多种血液分析物。然而，校准算法的预期应用并不成功，在该算法中，对独立样品的分析物浓度进行预测。这项可行性研究旨在重新评估近红外拉曼光谱的检测极限，并证明成功的校准转移。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Rapid and reliable measurements of the levels of clinically-relevant analytes are invaluable to physicians when making assessments about a patients treatment options. Current clinical laboratory tests involve sample preparation, the use of reagents, and are time-intensive. Raman spectroscopy is attractive in that it can measure multiple analytes at once with high specificty. In past research, NIR Raman has proven capable of detecting multiple blood analytes in the low millimolar range. However, prospective application of the calibration algorithm, in which analyte concentrations are predicted for independent samples, has not been successful. This feasibility study seeks to both re-evaluate NIR Raman spectroscopy detection limits and to demonstrate successful calibration transfer.