HYALURONAN-PROTEIN INTERACTIONS USING HYDROGEN/DEUTERIUM EXCHANGE MS

使用氢/氘交换 MS 分析透明质酸-蛋白质相互作用

• 批准号: 7601110
• 负责人: RON ORLANDO
• 金额: $ 28.22万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-02-01 至 2008-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7601110
• 关键词: Amides; Biological Phenomena; CD44 Antigens; Cell Adhesion; Computer Retrieval of Information on Scientific Projects Database; Deuterium; Deuterium Oxide; Extracellular Matrix; Funding; Grant; Hyaluronan; Hydrogen; Immune response; Institution; Ligands; Mass Spectrum Analysis; Methods; Monitor; Neoplasm Metastasis; Oligosaccharides; Proteins; Research; Research Personnel; Resources; Source; Surface; United States National Institutes of Health; Vertebral column; dalton; deuteron; insight; tool; tumor

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Protein-hyaluronan (HA) interactions are of profound importance in diverse biological phenomena such as extracellular matrix assembly, cell adhesion, tumor metastasis and the immune response. Therefore, tools for studying protein-HA interactions are essential to gain further insight into their function. Hydrogen/deuterium (H/D) amide-exchange mass spectrometry provides a powerful method for monitoring the free exchange of surface exposed amide hydrogens on a protein backbone. These amide hydrogens are labile and can be replaced by deuterons when exposed deuterium oxide results in an increase in 1 Dalton for each amide. To investigate protein-HA binding, the level of deuterium incorporation in the protein is analyzed in the absence and presence of the HA oligosaccharide ligand.

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