RELATIVE QUANTIFICATION OF STABLE ISOTOPE LABELED PEPTIDES

稳定同位素标记肽的相对定量

• 批准号: 7602131
• 负责人: John R Yates III
• 金额: $ 5.15万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7602131
• 关键词: Area; Biological; Brain; Chromosome Pairing; Complex; Computer Retrieval of Information on Scientific Projects Database; Coupled; Development; Funding; Grant; Hybrids; Institution; Ions; Label; Measurement; Numbers; Peptides; Performance; Proteomics; Range; Rattus; Relative (related person); Research; Research Personnel; Resolution; Resources; Sampling; Source; Stable Isotope Labeling; Standards of Weights and Measures; Synapses; Tissue Sample; United States National Institutes of Health; Work; comparative; mass analyzer; mass spectrometer; protein expression; research study; yeast protein

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The quantitative analysis of complex biological samples has emerged as a key research area in the field of proteomics. Although quantitative proteomic experiments remain challenging, these strategies have been greatly facilitated by the development of newer high-performance mass spectrometers. In this work, we have evaluated the use of the LTQ-Orbitrap, a hybrid mass spectrometer in which a linear ion trap is coupled to an Orbitrap mass analyzer, for quantitative analyses. By analyzing a range of yeast protein standards, we found that the high mass accuracy, high resolution, large ion capacity, and large dynamic range of the LTQ-Orbitrap led to as much as a 4-5-fold improvement in the number and quality of the peptide ratio measurements compared to similar analyses done on the LTQ. We also successfully quantified protein expression differences that occur in metabolically labeled rat synapses during brain development to further demonstrate the suitability of the LTQ-Orbitrap for the comparative analysis of complex tissue samples.

这个子项目是众多研究子项目之一