MOBILE PROTONS IN NEGATIVE CID TANDEM MS OF SULFATED OLIGOSACCHARIDES

硫酸低聚糖负 CID 串联质谱中的移动质子

• 批准号: 7602019
• 负责人: JOSEPH ZAIA
• 金额: $ 1.29万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-03 至 2008-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7602019
• 关键词: Carbohydrates; Chondroitin; Class; Computer Retrieval of Information on Scientific Projects Database; Data; Funding; Grant; Heating; Inorganic Sulfates; Institution; Ions; Laboratories; Oligosaccharides; Pattern; Polysaccharides; Process; Protons; Reaction; Research; Research Personnel; Resources; Sampling; Source; Structure; United States National Institutes of Health; Unspecified or Sulfate Ion Sulfates; Uronic Acids; Work; base; chondroitin sulfate glycosaminoglycan; sialylation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Carbohydrates of all classes consist of glycoform mixtures built on common core units. Determination of compositions and structures of such mixtures relies heavily on tandem mass spectrometric data. Native glycans are often preferred for samples available in very low quantities and for sulfated glycan classes. Negative tandem MS provides useful product ion profiles for neutral oligosaccharides and is preferred for acidic classes. In previous work from this laboratory, the influence of sialylation on product ion profiles in the negative mode was elucidated. The present work shows how the interplay of two other acidic groups, uronic acids and sulfates, determines product ion patterns for chondroitin sulfate glycosaminoglycan oligosaccharides. Unsulfated chondroitin oligosaccharides dissociate to form C-type ions almost exclusively. Sulfated forms produce only B- and Y-type ions. These observations are explained in terms of competing proton transfer reactions that occur during the collisional heating process. Mechanisms for product ion formation are proposed based on collisional breakdown diagrams of native and methyl esterified oligosaccharides.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 所有类别的碳水化合物都是由建立在共同核心单元上的糖型混合物组成。 这种混合物的组成和结构的测定在很大程度上依赖于串联质谱数据。 天然聚糖通常优选用于极少量可用的样品和硫酸化聚糖类别。 负串联质谱提供了中性寡糖的有用产物离子谱，并且优选用于酸性类别。 在本实验室先前的工作中，阐明了唾液酸化对负模式下产物离子分布的影响。 目前的工作表明，如何相互作用的其他两个酸性基团，糖醛酸和硫酸盐，决定产品离子模式硫酸软骨素糖胺聚糖寡糖。 未硫酸化的软骨素寡糖几乎完全解离形成C型离子。 硫酸盐形式仅产生B型和Y型离子。 这些观察结果解释在碰撞加热过程中发生的竞争质子转移反应。 基于天然和甲基酯化寡糖的碰撞分解图，提出了产物离子形成的机制。