DYNAMIC STRUCTURE OF L-O AND L-D PHASES IN SPM-DOPC-CHOLESTEROL BY ESR

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 为了将SPM/DOPC/胆固醇模型脂质体系的应用扩展到真实的质膜，用ESR波谱对相界面上的LO和LD相进行了动态表征，以与真实的质膜进行比较。通过用动力学模型对ESR谱进行拟合，可以得到有序参数和动力学参数，如旋转扩散系数，这些参数可以用来表征脂相。如果模型膜相的参数表现出与真实质膜中相同自旋探针参数相似的特征，那么我们可以肯定地说模型膜与真实膜相似。可以使用许多不同的膜自旋探针来增加表征的特异性以及增加对相态的理解。这一特征与LO+LD相共存区域的全局连接线构型相结合，将为脂筏提供更详细的模型。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. To expand the application of the SPM/DOPC/cholesterol model lipid system to real plasma membranes, the dynamic characterization of the Lo and Ld phases on the phase boundary by ESR spectra has been undertaken for comparison to real plasma membranes. The order parameter and dynamic parameters, such as rotational diffusion coefficients, can be obtained from fitting an ESR spectrum with a dynamic model, and these parameters can be used to characterize a lipid phase. If parameter from a model membrane phase exhibits similar characteristics to those parameters obtained from the same spin-probe in real plasma membranes, then we can say with confidence that the model membrane is similar to the real membrane. Many different membrane spin-probes can be employed to increase the specificity of the characterization as well as increasing the understanding of the phase state. This characterization combined with the global tie-line configuration for the Lo+Ld phase coexistence region will allow a more detailed model for lipid rafts.