CONFOCAL AND SPECIALIZED MICROSCOPY

共聚焦和专业显微镜共享资源是一个完全由癌症中心管理的设施。 亚细胞成像是癌症生物学中的重要工具。然而，虽然理想的标本， 传统的光学显微镜是二维的，而生物材料是三维组织的。 几种技术，特别是共聚焦显微镜，可用于收集生物的3D图像。 样品，具有优于常规显微镜的空间分辨率上级。这些基本的成像系统 对于没有经验的用户来说，操作和维护是昂贵和困难的，并且不是常规可用的 在个别研究实验室。共聚焦和专业显微镜提供的服务 共享资源是： 单光子激光扫描共聚焦显微镜 多光子激发共聚焦显微术 旋转圆盘共聚焦显微镜 数字去卷积显微术 所有这些三维荧光成像技术都是方便和经济的 在一个集中的设施中，可供CICC成员和其他哥伦比亚大学的研究人员使用， 由一名全职员工提供有关数据分析和演示的培训、辅助使用和咨询 还有一个经理自1996年以来，该设施已被超过154名来自世界各地的主要调查人员使用。 哥伦比亚大学的30个基础科学和临床部门，并为更多的人提供了图像 200篇文章该设施目前在高峰时段的使用率为最大容量的75%。在 2007年1月，该设施搬进了欧文癌症研究中心新装修的空间。的 我们不断寻求新的成像技术，以满足我们用户的科学需求。在 我们将在明年推出一种新的共聚焦显微镜。 在CCSG的最后一个阶段，37%的用户是癌症中心的成员， 这些成员占设施使用时间的26%。总 该设施的运营预算为273，025美元，其中我们要求CCSG提供79，749美元。

The Confocal and Specialized Microscopy Shared Resource is a fully Cancer Center-managed facility. Subcellular imaging is an essential tool in cancer biology. However, although the ideal specimen for conventional optical microscopy is two-dimensional, biological material is organized in three dimensions. Several technologies, notably confocal microscopy, are available to collect 3D images of biological samples, with a spatial resolution superior to conventional microscopy. These essential imaging systems are expensive and difficult for inexperienced users to operate and maintain, and are not routinely available in individual research laboratory. The services provided by the Confocal and Specialized Microscopy Shared Resource are: ¿ one-photon laser scanning confocal microscopy ¿ multiphoton excitation confocal microscopy ¿ spinning-disk confocal microscopy ¿ digital deconvolution microscopy All of these 3-dimensional fluorescence imaging technologies are made conveniently and affordably available to HICCC members and other Columbia University researchers in a centralized facility, with training, assisted use, and consultation on data analysis and presentation provided by a full-time employee and a manager. Since 1996, the facility has been used by more than 154 principal investigators from over 30 basic science and clinical departments at Columbia University, and has contributed images for more 200 publications. The facility is currently used at 75% of its maximal capacity during peak hours. In January 2007, the facility moved into newly renovated space in the Irving Cancer Research Center. The facility continually seeks to add new imaging technologies to meet the scientific needs of our users. In conjunction with the HICCC, we will introduce a new confocal microscope in the next year. During the last period of the CCSG, 37% of the users have been Cancer Center members with peerreviewed funding, with those members representing 26% of the hours used in the facilty. The total operating budget of the facility is $273,025, of which we are requesting $79,749 from the CCSG.