Anisotropic and Nonlinear Structure-Function of Normal and Degenerate Tendon

正常肌腱和退化肌腱的各向异性和非线性结构函数

• 批准号: 7575775
• 负责人: DAWN M ELLIOTT
• 金额: $ 30.58万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7575775
• 关键词: Affect; Aging; Anisotropy; Articular Range of Motion; Athletic; Attention; Automobile Driving; Basic Science; Behavior; Biochemical; Collagen Fiber; Complex; Connective Tissue; Data; Disease; Environment; Extracellular Matrix; Failure; Fiber; Human; Image; In Situ; Injury; Joints; Knowledge; Location; Magnetic Resonance Imaging; Measurement; Measures; Mechanics; Methods; Modality; Modeling; Normal tissue morphology; Pain; Physiological; Positioning Attribute; Process; Property; Research; Research Design; Role; Rotator Cuff; Sampling; Shoulder; Stretching; Structure; Structure-Activity Relationship; Tail; Tendon structure; Testing; Texture; Tissue Engineering; Tissues; Transgenic Mice; Workplace; base; design; disability; functional restoration; improved; injured; innovation; kinematics; macromolecule; mathematical model; mechanical behavior; novel; prevent; programs; repaired; restoration; soft tissue; success; supraspinatus muscle; therapy design; treatment strategy

DESCRIPTION (provided by applicant): Rotator cuff damage due to degeneration and/or injury is a common cause of significant pain and disability affecting the young and old alike in workplace, recreational, athletic, and aging environments. Different from most tendons, the supraspinatus tendon must regularly withstand a complex loading environment including multi-axial tension, compression, and shear, which makes study of this problem difficult. The complex loading is due to its anatomical structure, with non-uniaxial forces generated by the surrounding soft tissues, the coracoacromial arch, oblique insertion angles, and non-uniform fiber orientations. In order to understand, predict, prevent, and treat supraspinatus degeneration and injury, as well as to design tissue engineered replacement structures, it is critical to develop a complete understanding of the mechanical capability of this tissue in normal and altered conditions. Therefore, the objective of this research program is to rigorously evaluate anisotropic and nonlinear structure-function relationships in normal and degenerate supraspinatus tendons. Specifically, we propose the following 2 aims: Aim 1: Quantify nonlinear and anisotropic mechanical behavior and collagen fiber re-orientation of normal and degenerated human supraspinatus tendon under multiple loading modalities including: uniaxial tension in the fiber-aligned and transverse directions, biaxial tension, and planar shear. Quantify biochemical composition and histological grade at adjacent locations to the mechanical test samples. Aim 2: Develop and apply a nonlinear anisotropic constitutive model to predict tendon structure-function relationships. Explicitly incorporate fiber distribution and fiber re-orientation under load and the contribution of fiber stretch, extrafibrillar matrix, and fiber-matrix interactions to tendon function. Determine the model parameters using the biaxial and shear experimental data from Aim 1 and validate the model by predicting the uniaxial tension data. Correlate model parameters with composition measurements to complete the structure-composition-function quantification and test hypotheses for these relationships with region and degeneration. Knowledge of these relationships will provide important information towards understanding the fundamental mechanisms driving normal tissue adaptation and aging, disease processes and potential treatments, and the design of tissue engineered replacement structures. PUBLIC HEALTH RELEVANCE: Rotator cuff damage due to degeneration and/or injury is a common cause of significant pain and disability affecting the young and old alike in workplace, recreational, athletic, and aging environments. Treatment strategies aim to restore mechanical function; however, tendon anisotropic and nonlinear mechanical behaviors are not well established. Therefore, the objective of this research program is to determine quantitative anisotropic and nonlinear structure-function relationships in normal and degenerate supraspinatus tendons.

描述(由申请人提供)：由于退行性和/或损伤造成的肩袖损伤是在工作场所、娱乐、运动和老龄化环境中影响年轻人和老年人的重大疼痛和残疾的常见原因。与大多数肌腱不同的是，冈上肌腱需要经常承受包括多轴拉伸、压缩和剪切在内的复杂载荷环境，这给这一问题的研究带来了困难。复杂的载荷是由于其解剖结构所致，非单轴力来自周围的软组织、喙肩弓、倾斜的插入角和不均匀的纤维取向。为了了解、预测、预防和治疗冈上肌退行性变和损伤，以及设计组织工程化替代结构，对正常和改变条件下该组织的机械性能的全面了解是至关重要的。因此，本研究的目标是严格评估正常和退行性冈上肌腱的各向异性和非线性结构-功能关系。具体地说，我们提出了以下两个目标：目标1：量化正常和退变的人冈上肌腱在多种加载方式下的非线性和各向异性的力学行为和胶原纤维的重定向，包括：纤维取向和横向的单轴拉伸、双向拉伸和平面剪切。在与机械测试样品相邻的位置量化生化成分和组织学等级。目的2：建立并应用非线性各向异性本构模型来预测肌腱结构-功能关系。明确结合了负荷下的纤维分布和纤维重定向，以及纤维拉伸、纤维外基质和纤维-基质相互作用对肌腱功能的贡献。利用Aim 1的双轴和剪切试验数据确定模型参数，并通过预测单轴拉伸数据对模型进行验证。将模型参数与组成测量相关联，以完成结构-组成-功能的量化，并检验这些与区域和退化的关系的假设。对这些关系的了解将为理解推动正常组织适应和衰老的基本机制、疾病过程和潜在的治疗方法以及组织工程化替代结构的设计提供重要信息。 公共卫生相关性：由于退变和/或损伤造成的肩袖损伤是在工作场所、娱乐、运动和老龄化环境中影响年轻人和老年人的重大疼痛和残疾的常见原因。治疗策略旨在恢复肌腱的机械功能；然而，肌腱的各向异性和非线性力学行为还没有得到很好的确定。因此，本研究的目标是确定正常和退行性冈上肌腱的定量各向异性和非线性结构-功能关系。