Flow Cytometry

对于癌症中心的成员来说，流式细胞术是一项关键技术。流式细胞术可用于分析 单个细胞的光散射和荧光特性以及快速、统计、详细的分析 10000个细胞的数量。不是对定量信息进行平均，而是保留单个单元格的数据 亚群可以在多个维度上进行识别，这些细胞具有特定的荧光图谱 也可以收集(最多4个不同的组)用于生长或使用分选机进行进一步分析。纽约大学 癌症研究所流式细胞术设备为买不起自己的分选器和分析仪的用户提供 使用这项技术。该设施拥有一台达科MoFlo分拣机，配有3台激光和多达8种颜色的 它还具有两个Becton Dickinson多色分析仪，可以进行多色分析 由设施中的技术人员或直接由用户进行。经理和总监也参加了培训 活动包括个人咨询、公共研讨会和对用户的动手培训。该设施 在最近12个月期间进行了984小时的分析和585小时的分类。用户 在最近12个月中，使用可获得的分析仪804小时。

Flow cytometry is a critical technology for members of the Cancer Center. Flow cytometry allows analysis of the light scattering and fluorescence properties of individual cells and the rapid, statistically detailed analysis of 10,000s of cells. Rather than averaging the quantitative information the data on individual cells is retained and subpopulations can be identified in multiple dimensions, the cells with particular fluorescence profiles can also be collected (up to 4 different groups) for growth or further analysis using the sorter. The NYU Cancer Institute Flow Cytometry facility offers users who cannot afford their own Sorters and Analyzers access to this technology. The facility has a Dako MoFlo sorter with 3 lasers and up to 8 colors of fluorescence and it also has two Becton Dickinson analyzers for multicolor analysis, which can be performed by a technician in the facility or directly by users. The manager and director also participate in training activities including individual consultations, public seminars and hands on training of users. The facility performed 984 hours of analysis and 585 hours of sorting during the most recent 12 month period. The user accessible analyzer was used for 804 hours in the recent 12 month period.