Flow Cytometry

流式细胞术

• 批准号: 7714215
• 负责人: Michael Loran Dustin
• 金额: $ 5.42万
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-04-18 至 2013-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7714215
• 关键词: Arts; Cancer Center; Cell Separation; Cells; Clinical; Color; Consultations; Data; Flow Cytometry; Fluorescence; Growth; Hand; Hour; Individual; Institutes; Laboratories; Lasers; Malignant Neoplasms; Medical center; Patient Care; Policies; Postdoctoral Fellow; Property; Purpose; Research; Resources; Services; Sorting - Cell Movement; Students; Support of Research; System; Technology; Training; Training Activity; Training Technics; cell dimension; design; light scattering; member

Flow cytometry is a critical technology for members of the Cancer Center. Flow cytometry allows analysis of the light scattering and fluorescence properties of individual cells and the rapid, statistically detailed analysis of 10,000s of cells. Rather than averaging the quantitative information the data on individual cells is retained and subpopulations can be identified in multiple dimensions, the cells with particular fluorescence profiles can also be collected (up to 4 different groups) for growth or further analysis using the sorter. The NYU Cancer Institute Flow Cytometry facility offers users who cannot afford their own Sorters and Analyzers access to this technology. The facility has a Dako MoFlo sorter with 3 lasers and up to 8 colors of fluorescence and it also has two Becton Dickinson analyzers for multicolor analysis, which can be performed by a technician in the facility or directly by users. The manager and director also participate in training activities including individual consultations, public seminars and hands on training of users. The facility performed 984 hours of analysis and 585 hours of sorting during the most recent 12 month period. The user accessible analyzer was used for 804 hours in the recent 12 month period.

流式细胞仪是癌症中心成员的关键技术。流式细胞仪允许分析 单个细胞的光散射和荧光特性以及快速，统计详细的分析 10,000个细胞。保留了单个单元的数据，而不是平均定量信息 可以在多个维度上识别亚群，具有特定荧光曲线的细胞 也可以使用划落器收集（最多4个不同的组）进行生长或进一步分析。纽约大学 癌症研究所流式细胞仪设施为无法负担自己的分类器和分析仪的用户提供 访问这项技术。该设施有一个Dako Moflo分选仪，带有3个激光器，最多8种颜色 荧光，还具有两个Becton Dickinson分析仪进行多色分析，可以进行多色 由设施中的技术人员或用户直接由用户。经理和董事还参加培训 包括个人咨询，公开研讨会和动手培训用户的活动。设施 在最近的12个月期间，进行了984小时的分析和585小时的分类。用户 在最近的12个月期间，使用了804小时的可访问分析仪。