Muscarinic regulation of plasticity in the brain

毒蕈碱对大脑可塑性的调节

• 批准号: 7590495
• 负责人: GENE E ROBINSON
• 金额: $ 26.49万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7590495
• 关键词: Aging; Animals; Apis; Bees; Behavior; Behavioral; Biogenic Amines; Biological Assay; Biological Models; Brain; Brain Injuries; Candidate Disease Gene; Cells; Cholinergic Agents; Cholinergic Receptors; Coupled; Coupling; Dendrites; Development; Drosophila genus; Foundations; Future; Gene Expression; Genes; Genetic; Genome; Goals; Gold; Golgi Apparatus; Growth; Honey; Hormones; Human; In Situ Hybridization; Individual; Insecta; Investigation; Knowledge; Laboratories; Learning; Length; Letters; Link; Lobe; Longevity; Mediating; Memory; Methods; Microarray Analysis; Modeling; Molecular; Molecular Biology; Muscarinic Acetylcholine Receptor; Muscarinic Agonists; Muscarinic Antagonists; Muscarinics; Mushroom Bodies; Nervous System Physiology; Nervous system structure; Neuroanatomy; Neuronal Plasticity; Neurons; Neuropil; Nicotinic Agonists; Pathway interactions; Pattern; Performance; Pharmacology; Phenotype; Pilocarpine; Pollen; Process; Psyche structure; Public Health; Regulation; Research; Research Personnel; Reverse Transcriptase Polymerase Chain Reaction; Role; Sensory; Signal Transduction; Solid; Structure; Techniques; Testing; Translating; Urticaria; Work; base; body volume; brain volume; cholinergic; design; developmental psychobiology; experience; genome sequencing; improved; insight; mature animal; neurotransmission; neurotransmitter agonist; novel; postsynaptic; programs; research study; therapy development; tool

This project is designed to elucidate mechanisms that translate experience into changes in brain structure that allow adult animals to enhance their behavioral performance. Our model system, foraging-induced growth of the mushoom bodies (insect brain center for learning and memory) in the honey bee, permits nvestigations at the behavioral, cellular, and molecular levels. Our proposal is based on the surprising, but robust, demonstration that treatment of caged bees with a muscarinic agonist, pilocarpine, results in brain plasticity identical to that produced by a week of real foraging experience. We will: 1. determine how signaling via cholinergic pathways is related to foraging-induced increases in the volume of mushroom body neuropil using a novel experience-replacement technique; 2. determine the cellular phenotype of pilocarpine- induced changes in mushroom body neurons (Kenyon cells) using the Golgi technique; and 3. identify genes expressed in the mushroom bodies responsive to signaling via muscarinic pathways using whole bee genome microarrays, and then confirm and extend these results with quantitative RT-PCR and in situ hybridization. The bee provides a superb model system for these studies because appropriate tools, such as a sequenced genome, are now available, and because it is possible to rigorously manipulate the experience of the bee under naturalistic conditions and study effects at the neuroanatomical and molecular levels. The principal significance of this research is that it will reveal how experience is coupled to brain plasticity. Extensive conservation of nervous system function at the molecular level across the animal kingdom makes the results of our investigations on an insect broadly applicable within the field of behavioral development. This research is relevant to public health because experiments that can be efficiently performed using the simpler insect nervous system are likely to reveal how learning changes the brain in all animals, including humans. Such understanding is the first step in the development of therapies to improve human learning after brain damage. Our results will also suggest directions for the development of treatments for the decline in mental function that accompanies human aging.

该项目旨在阐明将经验转化为大脑结构变化的机制 使成年动物能够提高其行为表现。我们的模型系统，由觅食引起 蜜蜂蘑菇体（昆虫大脑学习和记忆中心）的生长，允许 行为、细胞和分子水平的研究。我们的提议是基于令人惊讶但 强有力的证据表明，用毒蕈碱激动剂毛果芸香碱治疗笼养蜜蜂会导致大脑 可塑性与一周真实觅食经验所产生的可塑性相同。我们将： 1. 确定如何 通过胆碱能途径的信号传导与觅食引起的蘑菇体体积增加有关 使用新颖的经验替换技术的神经纤维； 2. 确定毛果芸香碱的细胞表型- 使用高尔基体技术诱导蘑菇体神经元（凯尼恩细胞）的变化； 3. 识别基因 使用全蜂在蘑菇体中表达，对通过毒蕈碱途径发出的信号作出反应 基因组微阵列，然后通过定量 RT-PCR 和原位验证和扩展这些结果 杂交。蜜蜂为这些研究提供了一个极好的模型系统，因为适当的工具，例如 测序的基因组现在已经可用，并且因为可以严格操纵经验 在自然条件下蜜蜂的行为，并研究神经解剖学和分子水平的影响。这 这项研究的主要意义在于它将揭示经验如何与大脑可塑性相结合。 整个动物界在分子水平上广泛保护神经系统功能 我们对昆虫的调查结果广泛适用于行为发展领域。 这项研究与公共卫生相关，因为可以使用 更简单的昆虫神经系统可能会揭示学习如何改变所有动物的大脑，包括 人类。这种理解是开发改善人类学习疗法的第一步 脑损伤后。我们的结果还将为衰退治疗方法的开发提供方向 伴随人类衰老的心理功能。

项目成果

Muscarinic regulation of Kenyon cell dendritic arborizations in adult worker honey bees.

• DOI: 10.1016/j.asd.2011.01.003
• 发表时间: 2011-09
• 期刊: ARTHROPOD STRUCTURE & DEVELOPMENT
• 影响因子: 2
• 作者: Dobrin, Scott E.;Herlihy, J. Daniel;Robinson, Gene E.;Fahrbach, Susan E.
• 通讯作者: Fahrbach, Susan E.

What's the buzz about honeybee memory?

蜜蜂的记忆力有哪些热门话题？

• DOI: 10.1523/jneurosci.3951-10.2010
• 发表时间: 2010
• 期刊: The Journal of neuroscience : the official journal of the Society for Neuroscience
• 作者: Dobrin,ScottE;Wilson,DanielE
• 通讯作者: Wilson,DanielE

Visual associative learning in restrained honey bees with intact antennae.

• DOI: 10.1371/journal.pone.0037666
• 发表时间: 2012
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Dobrin SE;Fahrbach SE
• 通讯作者: Fahrbach SE