Surface Analysis Facility for Biomedical Problems

生物医学问题的表面分析设施

• 批准号: 7479570
• 负责人: DAVID G CASTNER
• 金额: $ 77.84万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-09-01 至 2009-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7479570
• 关键词: Address; Antibodies; Area; Arts; Biocompatible Materials; Biological; Biological Models; Biology; Biomedical Research; Biosensor; Carbohydrates; Cells; Chemicals; Chemistry; Communities; Complex; DNA; DNA Microarray Chip; DNA Microarray format; Data Analyses; Development; Devices; Educational workshop; Electrons; Film; Foundations; Frequencies; Generations; Gold; Health; Human; Image; Image Analysis; In Vitro; Information Resources; Investigation; Link; Lipids; Mechanics; Medical; Methods; Microbial Biofilms; Modems; Molecular; Molecular Structure; Multivariate Analysis; Nature; North America; Oligonucleotides; Paper; Pathway interactions; Peptides; Performance; Play; Postdoctoral Fellow; Protein Microchips; Proteins; Protocols documentation; Publications; Qualifying; Quantum Dots; Range; Research; Research Personnel; Research Project Grants; Resolution; Role; Scanning Probe Microscopy; Services; Site; Societies; Spatial Distribution; Spectrometry, Mass, Secondary Ion; Spectrum Analysis; Staging; Standards of Weights and Measures; Structure; Students; Sum; Surface; Surface Plasmon Resonance; Surface Properties; System; Techniques; Technology; Three-Dimensional Imaging; Time; Tissue Engineering; Training; Universities; Visit; Washington; Work; absorption; base; biomedical facility; cell growth; cellular imaging; day; design; implantable device; in vivo; instrument; instrumentation; interfacial; ion source; lectures; molecular recognition; monolayer; nanobiology; nanoparticle; nanotoxicity; physical property; programs; research and development; research study; spectroscopic imaging; technique development; training project

DESCRIPTION: (provided by applicant): NESAC/BIO provides the biomedical research community with state-of-the-art surface analysis expertise, instrumentation, experimental protocols, and data analysis methods to address biological and medical problems involving the surface and interfacial regions. Since the nature of the surface strongly influences the composition and recognizability of the biomolecules and cells that interact with materials, understanding the structure of surfaces and the nature of adsorbed biomolecules are key links in understanding interfacial biology for both in vivo (e.g., implanted biomedical devices) and in vitro (e.g., DNA microarrays) applications. ESCA, static SIMS, NEXAFS, SFG and SPM, provide a powerful set of complementary techniques for addressing the challenges and complexity of the new generation of biomaterials and devices being developed with molecular recognition principles. The specific aims of the Technology Research and Development projects are: (1) Surface Analysis Model Systems and Standards, (2) Chemical State Imaging, and (3) Instrument and Technique Development. In the next five years there will be an increased effort at NESAC/BIO on developing instrumentation and techniques for the surface characterization of biosensors (DNA microarrays, protein chips, etc.). Multivariate analysis methods will play an important role in both spectroscopic and image analysis. Standards used in the development of these techniques will include self-assembled monolayers, peptides, and oligonucleotides. The specific aim of the Collaborative Research projects is to use the surface analysis techniques and methods developed in the TRD projects to address important biomedical research problems. The areas of investigation include biosensors, nanobiology, tissue engineering, self-assembled films, cell growth surfaces with peptides, lipid surfaces, DNA arrays, and biomaterials with specific recognition sites. NESAC/BIO service activities will focus on the development and optimization of biomedical devices. Dissemination and Training projects will also be an important part of the NESAC/BIO program.

描述：（由申请人提供）：NESAC/BIO为生物医学研究界提供最先进的表面分析专业知识，仪器，实验协议和数据分析方法，以解决涉及表面和界面区域的生物学和医学问题。由于表面的性质强烈影响与材料相互作用的生物分子和细胞的组成和可识别性，因此了解表面的结构和吸附生物分子的性质是了解体内（例如植入的生物医学设备）和体外（例如DNA微阵列）应用的界面生物学的关键环节。ESCA，静态SIMS， NEXAFS， SFG和SPM提供了一套强大的互补技术，用于解决新一代生物材料和设备的挑战和复杂性，这些材料和设备正在利用分子识别原理开发。技术研发项目的具体目标是：(1)表面分析模型系统和标准；(2)化学状态成像；(3)仪器和技术开发。在未来五年内，NESAC/BIO将加大力度开发用于生物传感器（DNA微阵列、蛋白质芯片等）表面表征的仪器和技术。多变量分析方法将在光谱分析和图像分析中发挥重要作用。这些技术开发中使用的标准将包括自组装单层、多肽和寡核苷酸。合作研究项目的具体目标是利用TRD项目中开发的表面分析技术和方法来解决重要的生物医学研究问题。研究领域包括生物传感器、纳米生物学、组织工程、自组装膜、多肽细胞生长表面、脂质表面、DNA阵列和具有特定识别位点的生物材料。NESAC/BIO服务活动将侧重于生物医学设备的开发和优化。传播和培训项目也将是NESAC/BIO计划的重要组成部分。