ION CHANNEL STRUCTURE AND MECHANISM

离子通道结构和机制

• 批准号: 8362549
• 负责人: Michael H. B. Stowell
• 金额: $ 2.13万
• 依托单位: UNIVERSITY OF COLORADO
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362549
• 关键词: Action Potentials; Affinity Chromatography; Calcium Channel; Cell physiology; Cellular Structures; Computer software; Crystallography; Detection; Electron Microscopy; Elements; Funding; Gated Ion Channel; Goals; Grant; Ion Channel; Ligands; Membrane; Methods; Molecular; National Center for Research Resources; Preparation; Principal Investigator; Prokaryotic Cells; Research; Research Infrastructure; Resources; Signal Transduction; Source; Structure; System; Tomogram; United States National Institutes of Health; cost; interest; programs; voltage gated channel

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Several ion channels are being studied with the plan of determining the molecular mechanism of their action. These include voltage gated channels responsible for propagation and termination of action potentials, calcium channels involved in signal amplification and ligand gated ion channels involved in signal detection and modulation. Using rapid affinity purification methods, along with x-ray crystallography and electron microscopy, our goal is to elucidate the structural elements of these channels in various state. Systems of current interest include the prokaryotic mechanosensitive channels (Msc), including those of large (MscL) and small (MscS) conductance that couple channel gating with membrane tension. We have begun to collect cryo-tomograms of membrane preparations of prokaryotes that contain mechanosensitive channels and are applying 3D volume averaging using out PEET software program.

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 目前正在研究几种离子通道，并计划确定其作用的分子机制。这些通道包括负责动作电位传播和终止的电压门控通道、参与信号放大的钙通道和参与信号检测和调制的配基门控离子通道。利用快速亲和纯化方法，结合X射线结晶学和电子显微镜，我们的目标是阐明这些通道在不同状态下的结构元素。目前感兴趣的系统包括原核生物机械敏感通道(MSC)，包括那些耦合通道门控和膜张力的大(MSCL)和小(MSCs)电导的通道。我们已经开始收集含有机械敏感通道的原核生物膜制剂的冷冻断层图像，并正在使用OUT Peet软件程序进行三维体积平均。