Membrane Protein Polyhedral Nanoparticles for High Resolution Structure/Function Studies

用于高分辨率结构/功能研究的膜蛋白多面体纳米颗粒

• 批准号: 9136219
• 负责人: Michael H. B. Stowell
• 金额: $ 30.42万
• 依托单位: UNIVERSITY OF COLORADO
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-10 至 2017-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9136219
• 关键词: Bacteriorhodopsins; Biological; Capsid; Chloroplasts; Choline; Complex; Cryoelectron Microscopy; Crystallography; Data Set; Detergents; Drug Prescriptions; Dyes; Electron Microscope; Electron Microscopy; Electrons; Environment; Freezing; G-Protein-Coupled Receptors; Gated Ion Channel; Harvest; Health; Ions; Ligands; Light; Lipids; Measures; Membrane; Membrane Lipids; Membrane Potentials; Membrane Proteins; Methods; Microfluidic Microchips; Microfluidics; Molecular Conformation; Problem Solving; Protein Analysis; Proteins; Reporting; Resolution; Role; Specimen; Structure; Surface; Testing; Tube; Vesicle; Virion; Virus; Work; beta-2 Adrenergic Receptors; design; detector; insight; nanometer; nanoparticle; new therapeutic target; novel strategies; particle; protein structure; proteoliposomes; receptor; screening; self assembly; signal processing; stem; tool

DESCRIPTION (provided by applicant): This proposal aims to study the structure and function of membrane proteins using a novel approach that combines a microfluidic membrane self-assembling method with high throughput electron microscopy single particle analysis. We will leverage our previous work to realize a systematic approach to structural analysis of membrane proteins in a biological membrane. The overall objective of this proposal will be determining the structures of several membrane proteins both in the absence and presence of ligands and transmembrane gradients that alter the activity of the membrane protein.

描述（由申请人提供）：本提案旨在使用一种将微流控膜自组装方法与高通量电子显微镜单颗粒分析相结合的新方法来研究膜蛋白的结构和功能。我们将利用我们之前的工作来实现生物膜中膜蛋白结构分析的系统方法。该提案的总体目标是确定在不存在和存在配体以及改变膜蛋白活性的跨膜梯度的情况下几种膜蛋白的结构。