ION CHANNEL STRUCTURE AND MECHANISM

离子通道结构和机制

• 批准号: 8170847
• 负责人: Michael H. B. Stowell
• 金额: $ 3.74万
• 依托单位: UNIVERSITY OF COLORADO
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170847
• 关键词: Action Potentials; Affinity Chromatography; Calcium Channel; Computer Retrieval of Information on Scientific Projects Database; Computer software; Crystallography; Detection; Electron Microscopy; Elements; Funding; Gated Ion Channel; Goals; Grant; Institution; Ion Channel; Ligands; Membrane; Methods; Molecular; Preparation; Prokaryotic Cells; Research; Research Personnel; Resources; Signal Transduction; Source; Structure; System; Tomogram; United States National Institutes of Health; interest; programs; voltage gated channel

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Several ion channels are being studied with the plan of determining the molecular mechanism of their action. These include voltage gated channels responsible for propagation and termination of action potentials, calcium channels involved in signal amplification and ligand gated ion channels involved in signal detection and modulation. Using rapid affinity purification methods, along with x-ray crystallography and electron microscopy, our goal is to elucidate the structural elements of these channels in various state. Systems of current interest include the prokaryotic mechanosensitive channels (Msc), including those of large (MscL) and small (MscS) conductance that couple channel gating with membrane tension. We have begun to collect cryo-tomograms of membrane preparations of prokaryotes that contain mechanosensitive channels and are applying 3D volume averaging using out PEET software program.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 目前正在研究几种离子通道，并计划确定其作用的分子机制。这些包括负责动作电位的传播和终止的电压门控通道、参与信号放大的钙通道和参与信号检测和调节的配体门控离子通道。利用快速亲和纯化方法，沿着X射线晶体学和电子显微镜，我们的目标是阐明这些通道在不同状态下的结构元件。当前感兴趣的系统包括原核机械敏感通道（Msc），包括将通道门控与膜张力耦合的大电导（MscL）和小电导（MscS）的那些。我们已经开始收集含有机械敏感通道的原核生物膜制备物的冷冻断层图像，并使用PEET软件程序应用3D体积平均。