Membrane Protein Polyhedral Nanoparticles for High Resolution Structure/Function Studies

用于高分辨率结构/功能研究的膜蛋白多面体纳米颗粒

• 批准号: 8863303
• 负责人: Michael H. B. Stowell
• 金额: $ 30.33万
• 依托单位: UNIVERSITY OF COLORADO
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-10 至 2017-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8863303
• 关键词: Adrenergic Receptor; Bacteriorhodopsins; Biological; Capsid; Chloroplasts; Choline; Complex; Cryoelectron Microscopy; Crystallography; Data Set; Detergents; Drug Prescriptions; Dyes; Electron Microscope; Electron Microscopy; Electrons; Environment; Freezing; G Protein-Coupled Receptor Genes; Gated Ion Channel; Harvest; Health; Ions; Ligands; Light; Lipids; Measures; Membrane; Membrane Lipids; Membrane Potentials; Membrane Proteins; Methods; Microfluidic Microchips; Microfluidics; Molecular Conformation; Pharmaceutical Preparations; Principal Investigator; Problem Solving; Protein Analysis; Proteins; Reporting; Resolution; Role; Specimen; Structure; Surface; Testing; Tube; Vesicle; Virion; Virus; Work; beta-2 Adrenergic Receptors; design; detector; insight; nanometer; nanoparticle; novel strategies; particle; programs; protein structure; proteoliposomes; receptor; screening; self assembly; signal processing; stem; tool

 DESCRIPTION (provided by applicant): This proposal aims to study the structure and function of membrane proteins using a novel approach that combines a microfluidic membrane self-assembling method with high throughput electron microscopy single particle analysis. We will leverage our previous work to realize a systematic approach to structural analysis of membrane proteins in a biological membrane. The overall objective of this proposal will be determining the structures of several membrane proteins both in the absence and presence of ligands and transmembrane gradients that alter the activity of the membrane protein. 0925-0001/0002 (Rev. 08/12) Page Continuation Format Page

 描述（由申请人提供）：该提案旨在使用一种新方法研究膜蛋白的结构和功能，该方法将微流体膜自组装方法与高通量电子显微镜单颗粒分析相结合。我们将利用我们以前的工作，实现一个系统的方法来分析生物膜中的膜蛋白的结构。本提案的总体目标将是确定几种膜蛋白在不存在和存在改变膜蛋白活性的配体和跨膜梯度的情况下的结构。0925-0001/0002（2012年8月修订版）续页格式页