ION CHANNEL STRUCTURE AND MECHANISM

离子通道结构和机制

• 批准号: 7955070
• 负责人: Michael H. B. Stowell
• 金额: $ 3.22万
• 依托单位: UNIVERSITY OF COLORADO
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7955070
• 关键词: Action Potentials; Affinity Chromatography; Calcium Channel; Cellular Structures; Computer Retrieval of Information on Scientific Projects Database; Computer software; Crystallography; Detection; Electron Microscopy; Elements; Funding; Gated Ion Channel; Goals; Grant; Institution; Ion Channel; Ligands; Membrane; Methods; Molecular; Preparation; Prokaryotic Cells; Research; Research Personnel; Resources; Signal Transduction; Source; Structure; System; Tomogram; United States National Institutes of Health; interest; programs; voltage gated channel

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Several ion channels are being studied with the plan of determining the molecular mechanism of their action. These include voltage gated channels responsible for propagation and termination of action potentials, calcium channels involved in signal amplification and ligand gated ion channels involved in signal detection and modulation. Using rapid affinity purification methods, along with x-ray crystallography and electron microscopy, our goal is to elucidate the structural elements of these channels in various state. Systems of current interest include the prokaryotic mechanosensitive channels (Msc), including those of large (MscL) and small (MscS) conductance that couple channel gating with membrane tension. We have begun to collect cryo-tomograms of membrane preparations of prokaryotes that contain mechanosensitive channels and are applying 3D volume averaging using out PEET software program.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 正在研究几个离子通道的计划，以确定其作用的分子机制。其中包括负责传播和终止作用电位的电压门控通道，涉及信号扩增的钙通道以及参与信号检测和调节的配体门控离子通道。使用快速亲和力纯化方法以及X射线晶体学和电子显微镜，我们的目标是阐明各个状态下这些通道的结构元素。当前感兴趣的系统包括实质性机械敏感通道（MSC），包括大型（MSCL）和小型（MSC）电导的系统，这些电导与膜张力通道通道通道通道。我们已经开始收集包含机械敏感通道的原核生物的膜制剂的冷冻组合图，并正在应用使用PEET软件程序的3D卷。