BIOMARKERS OF BRAIN PATHOLOGY

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 该项目旨在开发一种使用液相色谱-质谱仪的协议，以量化使用猴子模型和使用小鼠模型实验诱导的更年期可卡因滥用引起的脑化学变化。在本年度，从自身服用可卡因的猴子的纹状体内收集的微透析样本中测量了谷氨酸和GABA的水平。 在第二个项目中，在对照组小鼠和过度表达淀粉样蛋白的小鼠中，诱导了卵巢功能衰竭，以模拟更年期特征的卵泡丢失。 在接下来的一年中，将对对照动物和实验动物的神经病理学发展进行分析。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project is designed to develop a protocols using liquid chromatography-mass spectroscopy to quantify changes in brain chemistry resulting from cocaine abuse using a monkey model and experimentally induced menopause using a mouse model. During the current year, levels of glutamate and GABA have been measured in microdialysis samples collected from the striatum of monkeys self-administering cocaine. In the second project, ovarian failure, to mimic the loss follicles characteristic of menopause, was induced in control mice and mice over-expressing the amyloid protein. Analyses will proceed in the coming year in the development of neuropathology in control versus experimental animals.