Laboratory Support

目标 为CIPRA项目提供实验室支持 在艾滋病毒-NAT为能力有限的地点提供中央实验室设施 在可能的情况下发展区域地点的实验室能力 制定QA/QC计划，以监控实验室间和实验室内的变异性，并使实验室程序标准化 用于专门的研究终点分析 提供地区性的药学水平良好实验室操作规程的培训计划 技术与质量管理 具体目标 根据项目增加的活动雇用额外的技术人员 购买额外的实验室设备，以适应与项目相关的更多活动 采购额外的管理基础设施来支持这个实验室核心 扩展现有的实验室设施，以适应与CIPRA计划相关的额外活动 方法 将雇用两名全职实验室技术员和三名助理实验室技术员。这个号码是 根据艾滋病毒-NAT实验室雇用的当前工作量分配公式。一间实验室 行政助理将受聘监督四个项目的文书工作。因为HIV-NAT将成为 多个地点的中心实验室，需要离心机、生物安全柜和流式细胞仪 预期活动增加。其他现有的设备是足够的。 所有四个项目的长期标本存储都需要额外的-80度冰柜。据设想，大多数 长期储存将发生在艾滋病毒-NAT。一种使用地面在站点和HIV-NAT之间运送标本的系统 由于固体碳供应不可靠，将发展使用便携式液氮储罐的航空运输 偏远地区的二氧化碳(干冰)。在装运之前，需要在区域地点进行临时的本地储存。 为此目的，将购买用于临时标本存储的小型冰柜。

Objectives To provide laboratory support for this CIPRA program To provide central laboratory facilities at HIV-NAT for those sites with limited capacity To develop laboratory capacity in regional sites where possible To develop QA/QC programs to monitor inter- and intra-laboratory variability and to standardize laboratory procedures for specialized study endpoint assays To provide regional training program in principles of pharmaceutical level Good Laboratory Practice, laboratory techniques and quality management Specific aims To employ additional technical personnel in accordance with increased activity according to the projects To purchase additional laboratory equipment to accommodate the increased activity associated with the projects To procure additional administrative infrastructure to support this laboratory core To expand the existing laboratory facilities to accommodate additional activity associated with the CIPRA program Methods Two full-time laboratory technologists and three assistant laboratory technicians will be employed. This number is based on the current formula for workload distribution for employment at the HIV-NAT laboratory. A laboratory administrative assistant will be employed to oversee the clerical aspects of the four projects. As HIV-NAT will be the central laboratory for many sites, the centrifuge, biological safety cabinet and flow cytometer are needed with the expected increase in activity. Other existing equipment is adequate. Additional -80 deg freezers will be needed for-long term specimen storage for all four projects. It is envisaged that most long-term storage will occur at HIV-NAT. A system of specimen transport between the sites and HIV-NAT using ground and air transport with portable liquid nitrogen tanks will be developed due to the unreliable supply of solid carbon dioxide (dry ice) in remote sites. There will be a need for temporary local storage at regional sites prior to shipment. Small freezers for temporary specimen storage will be purchased for this purpose.