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Quantitative Mass Spectrometer for Targeted and Global Proteomics, Metabolomics

用于靶向和整体蛋白质组学、代谢组学的定量质谱仪

基本信息

批准号：
8447773
负责人：
THOMAS G GRAEBER
金额：
$ 59.32万
依托单位：
UNIVERSITY OF CALIFORNIA LOS ANGELES
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-07-01 至 2014-06-30
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): A state-of-the-art hybrid tandem mass spectrometer (MS/MS) equipped with High Performance Liquid Chromatography (HPLC) and autosampler for quantitative assays of biological samples is requested. The instrument is chosen to support the thematic research of UCLA investigators using proteomics and metabolomics to study human health and disease. This instrument will be a vital component to support the specific aims of a broad range of NIH-funded projects. The projects involve the measurement of a variety of molecular species, including peptides digested from proteins associated with immune cells, intestinal lavage samples, core metabolic pathways such as glycolysis, lipids associated with biosynthetic pathways, inflammation response, and dysfunctional HDL. The proposed mass spectrometer system will be supported and administered by the UCLA Molecular Instrumentation Center (MIC), a campus-wide, integrated facility formed to enhance the accessibility of existing shared, sophisticated instrumentation facilities to the broader research community at the institution. Although triple quadrupole mass spectrometers excel over most other mass spectrometry designs in their ability to perform high throughput quantitative analysis of complex mixtures over a large dynamic range, we are requesting a newly developed hybrid high resolution quadrupole Orbitrap, the Q-Exactive. The Q-Exactive offers choices of scan functions amenable to quantitation not available to traditional triple quadrupole instruments, and offers versatility and flexibility in method development crucial to a core facility. In discovery applications, the Orbitrap analyzer, with its high mass accuracy and high resolution, can reduce the candidate identifications by five-fold or more. The dynamic range, greater than 4 orders of magnitude, and sensitivity are comparable to that achieved on current triple quad instrumentation.

描述（由申请人提供）：要求最先进的混合串联质谱仪（MS/MS），配备高效液相色谱（HPLC）和自动进样器，用于生物样品的定量分析。选择该仪器是为了支持加州大学洛杉矶分校研究人员利用蛋白质组学和代谢组学研究人类健康和疾病的专题研究。这一工具将是支持美国国立卫生研究院资助的广泛项目的具体目标的重要组成部分。这些项目涉及多种分子种类的测量，包括与免疫细胞相关的蛋白质消化的肽、肠道灌洗样品、糖酵解等核心代谢途径、与生物合成途径相关的脂质、炎症反应和功能失调的HDL。提议的质谱仪系统将由加州大学洛杉矶分校分子仪器中心（MIC）支持和管理，这是一个校园范围内的综合设施，旨在提高现有共享的复杂仪器设备对该机构更广泛的研究社区的可访问性。虽然三重四极杆质谱仪在大动态范围内对复杂混合物进行高通量定量分析的能力优于大多数其他质谱设计，但我们需要一种新开发的混合高分辨率四极杆Orbitrap， Q-Exactive。Q-Exactive提供了传统三重四极杆仪器无法提供的定量扫描功能的选择，并提供了对核心设施至关重要的方法开发的通用性和灵活性。在发现应用中，Orbitrap分析仪具有高质量精度和高分辨率，可以将候选识别减少五倍或更多。动态范围，大于4个数量级，灵敏度可与当前的三重四轴仪器相媲美。