Quantitative Mass Spectrometer for Targeted and Global Proteomics, Metabolomics

描述(由申请人提供)：需要一台最先进的混合串联质谱仪(MS/MS)，配备高效液相色谱(HPLC)和自动进样器，用于生物样品的定量分析。选择该仪器是为了支持加州大学洛杉矶分校研究人员使用蛋白质组学和代谢组学研究人类健康和疾病的主题研究。这项文书将是支持国家卫生研究院资助的广泛项目的具体目标的重要组成部分。这些项目涉及各种分子种类的测量，包括从与免疫细胞相关的蛋白质中消化的多肽、肠道洗液样本、核心代谢途径(如糖酵解)、与生物合成途径相关的脂类、炎症反应和功能失调的高密度脂蛋白。拟议的质谱仪系统将由加州大学洛杉矶分校分子仪器中心(MIC)支持和管理，该中心是一个校园范围的综合设施，旨在提高现有共享的、复杂的仪器设施对该机构更广泛的研究社区的可访问性。尽管三重四极质谱计在大动态范围内对复杂混合物进行高通量定量分析的能力优于大多数其他质谱仪设计，但我们需要一种新开发的混合型高分辨率四极轨道探测器Q-Exactive。Q-Exactive提供了传统三重四极杆仪器无法提供的定量扫描功能选择，并在方法开发方面提供了对核心设施至关重要的多功能性和灵活性。在发现应用中，Orbitrap分析仪具有高质量精度和高分辨率，可以将候选识别减少五倍或更多。其动态范围大于4个数量级，灵敏度可与目前的三重四通道仪器相当。

DESCRIPTION (provided by applicant): A state-of-the-art hybrid tandem mass spectrometer (MS/MS) equipped with High Performance Liquid Chromatography (HPLC) and autosampler for quantitative assays of biological samples is requested. The instrument is chosen to support the thematic research of UCLA investigators using proteomics and metabolomics to study human health and disease. This instrument will be a vital component to support the specific aims of a broad range of NIH-funded projects. The projects involve the measurement of a variety of molecular species, including peptides digested from proteins associated with immune cells, intestinal lavage samples, core metabolic pathways such as glycolysis, lipids associated with biosynthetic pathways, inflammation response, and dysfunctional HDL. The proposed mass spectrometer system will be supported and administered by the UCLA Molecular Instrumentation Center (MIC), a campus-wide, integrated facility formed to enhance the accessibility of existing shared, sophisticated instrumentation facilities to the broader research community at the institution. Although triple quadrupole mass spectrometers excel over most other mass spectrometry designs in their ability to perform high throughput quantitative analysis of complex mixtures over a large dynamic range, we are requesting a newly developed hybrid high resolution quadrupole Orbitrap, the Q-Exactive. The Q-Exactive offers choices of scan functions amenable to quantitation not available to traditional triple quadrupole instruments, and offers versatility and flexibility in method development crucial to a core facility. In discovery applications, the Orbitrap analyzer, with its high mass accuracy and high resolution, can reduce the candidate identifications by five-fold or more. The dynamic range, greater than 4 orders of magnitude, and sensitivity are comparable to that achieved on current triple quad instrumentation.