Expanding the toolbox for tsetse reproductive biology

扩展采采蝇生殖生物学的工具箱

• 批准号: 8622915
• 负责人: Serap AKSOY
• 金额: $ 24.17万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-01-06 至 2015-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8622915
• 关键词: Affect; Africa South of the Sahara; African Trypanosomiasis; Animal Model; Animals; Behavior; Behavioral; Biology; Complex; Computer Simulation; Data; Data Set; Deposition; Development; Disease; Disease Vectors; Disease model; Evolution; Female; Fertility; Fluorescent in Situ Hybridization; Foundations; Future; Gene Expression; Gene Expression Profile; Gene Proteins; Gene Silencing; Genome; Genomics; Gland; Goals; Human; Insecta; Knowledge; Longevity; Mediating; Methods; Molecular Genetics; Molecular Profiling; Nucleic Acid Regulatory Sequences; Oogenesis; Organism; Orthologous Gene; Partner in relationship; Pattern; Pesticides; Physiological; Physiology; Play; Polymorphism Analysis; Population; Population Sizes; Proteins; Proteomics; Protocols documentation; Regulation; Regulatory Element; Reproduction; Reproductive Biology; Reproductive Physiology; Research; Resources; Role; Sampling; Seminal fluid; Set protein; Specificity; Spermatogonia; Study models; Synteny; Techniques; Tissues; Toxic effect; Trypanosomiasis; Tsetse Flies; base; comparative; cost; disease transmission; egg; fitness; fly; knowledge base; male; neglect; non-genetic; novel; offspring; paralogous gene; prevent; protein function; public health relevance; reproductive; sex; sperm cell; tool; transcriptomics; transmission process; vector control; young adult

Project Summary/Abstract Tsetse flies transmit the human and animal forms of African Trypanosomiasis, neglected diseases, which affect marginalized populations in sub-Saharan Africa. Control methods based upon pesticide-baited targets and traps can reduce tsetse populations, but have downsides, including environmental toxicity, collateral effects upon non- target organisms and high implementation cost. Thus, genetic and non-pesticide based vector control methods are desirable. This project will build the foundation for control methods that aim to reduce tsetse's reproductive capacity. We will investigate male accessory gland (MAGs) products, known as seminal fluid proteins (SFPs), which are transferred along with sperm to the female during mating. SFPs mediate important physiological and behavioral changes in mated female insects, including reduced receptivity to remating and increased oogenesis/egg deposition. Disruption/manipulation of SFP function(s) has the potential to reduce tsetse populations and hence disease transmission. Tsetse flies have a low reproductive capacity as they reproduce by obligate viviparity. Each gonotrophic cycle, females carry and nourish a single intrauterine offspring for the duration of its development. Females on average produce 8-10 offspring per lifetime. Our proposed studies are supported by the availability of 1) preliminary transcriptomic data on MAG gene expression in our target species Glossina morsitans morsitans (Gmm), 2) an annotated genome and extensive transcriptomic resources for Gmm, and 3) a large dataset of transcriptomes from other tsetse species. This study has 3 aims: 1. Identify putative SFP genes using transcriptomic, proteomic and genomic approaches, characterize their sex/tissue specificity, and examine temporal expression profiles during maturation and after mating. 2. Identify SFP orthologs and paralogs between multiple Glossina species by utilizing available transcriptomic and genomic resources, estimate sequence diversity of SFPs by polymorphism detection and identify conserved sex and tissue specific regulatory elements by in silico analysis. 3. Develop and apply gene silencing techniques to assess the functional role(s) of selected SFPs (~5) within the context of the tsetse reproductive fitness parameters.

项目总结/摘要 采采蝇传播人类和动物形式的非洲锥虫病，这种被忽视的疾病， 撒哈拉以南非洲的边缘化人口。基于农药诱饵靶标和诱捕器的控制方法 可以减少采采蝇的数量，但也有不利的一面，包括对环境的毒性， 且实施成本高。因此，基于基因和非农药的病媒控制方法 是可取的。该项目将为旨在减少采采蝇繁殖的控制方法奠定基础。 容量我们将研究男性附属腺（MAG）的产物，称为精液蛋白（SFP）， 在交配过程中，精子沿着转移到雌性体内。SFPs介导重要的生理和 交配后的雌性昆虫的行为变化，包括对再交配的接受性降低， 卵子发生/卵子沉积。破坏/操纵SFP功能有可能减少采采蝇 人口和疾病传播。采采蝇的繁殖能力很低，因为它们通过 专性胎生每个生殖营养周期，雌性携带并养育一个子宫内的后代， 其发展的持续时间。雌性平均一生生育8-10个后代。我们建议的研究是 支持的可用性1）初步转录组学数据MAG基因表达在我们的目标物种 2）Gmm的注释基因组和广泛的转录组学资源， 和3）来自其他采采蝇物种的转录组的大数据集。本研究有三个目的： 1. 使用转录组学、蛋白质组学和基因组学方法鉴定推定的SFP基因， 性别/组织特异性，并检查在成熟过程中和交配后的时间表达谱。 2. 通过利用可用的转录组学鉴定多个Glossina物种之间的SFP直系同源物和旁系同源物 和基因组资源，通过多态性检测估计SFPs的序列多样性， 通过计算机分析保守的性别和组织特异性调控元件。 3. 开发和应用基因沉默技术，以评估选定的SFPs（~5）在 采采蝇生殖适合度参数的背景。