ShEEP request for an Inscopix nVoke Integrated Imaging and Optogenetics System

ShEEP 请求 Inscopix nVoke 集成成像和光遗传学系统

• 批准号: 9795729
• 负责人: Karunesh Ganguly
• 金额: --
• 依托单位: VETERANS AFFAIRS MED CTR SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-01-01 至 2019-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9795729
• 关键词: Animals; Behavior; Behavioral; Brain; Cells; Code; Computer software; Contracts; Corpus striatum structure; Data; Development; Dissection; Environment; Equipment; Floor; Head; Hippocampus (Brain); Image; Implant; Implantation procedure; Individual; Injury; Intervention; Investigation; Knowledge; Label; Learning; Location; Maintenance; Memory; Microscope; Monitor; Nervous system structure; Neurofibrillary Tangles; Neurons; Pattern; Phase; Process; Recovery; Research; Resolution; Route; Sheep; Signal Transduction; Stroke; Structure; Survivors; System; Technology; Therapeutic Intervention; Time; Traumatic Brain Injury; United States; Veterans; Work; awake; cell type; cost; data acquisition; disability; equipment acquisition; flexibility; insight; lens; neurotransmission; new technology; novel therapeutics; optogenetics; relating to nervous system

Stroke and traumatic brain injury are leading causes of disability among veterans. While there has been substantial research into understanding mechanisms of recovery, there is a relative dearth of studies that delineate the contributions of specific cell types, e.g. excitatory and/or inhibitory subtypes. Such knowledge provides greater insight into the recovery process and can aid the development of targeted interventions. We request the purchase of the Inscopix nVoke Integrated Imaging and Optogenetics System to conduct such investigations. The Inscopix miniature microscope platform gives us an edge in investigating how patterned activity in neuronal ensembles represents information about an individual’s environment, actions, and memories. A key advantage of this system is the ability to image deep neural structures (e.g. striatum, hippocampus) through a flexible scope during freely moving behaviors. Importantly, by monitoring and disrupting activity in the same cells over months, we can gain unprecedented insights into how neural codes evolve during recovery and with therapeutic interventions. A critical aspect of this technology is unprecedented monitoring at a single-cell resolution of identified cell-types; this represents the critical next phase of circuit function dissection.

中风和创伤性脑损伤是退伍军人致残的主要原因。虽然已经有了 虽然对恢复机制的理解进行了大量研究，但相对缺乏的研究 描述特定细胞类型的贡献，例如兴奋性和/或抑制性亚型。是这样的 知识可以更好地洞察恢复过程，并有助于制定有针对性的 干预措施。我们请求购买Inscope ix nVoke集成成像和光遗传学 进行这类调查的系统。Inscope ix微型显微镜平台使我们在 研究神经元群中的模式活动如何代表关于个体的信息 环境、行动和记忆。该系统的一个关键优势是能够对深层神经进行成像。 结构(如纹状体、海马体)在自由活动时通过一个灵活的范围。 重要的是，通过监测和扰乱相同细胞数月来的活动，我们可以获得 对神经代码在康复期间和治疗干预过程中如何演变的前所未有的见解。 这项技术的一个关键方面是以前所未有的单细胞分辨率监测已识别的 细胞类型；这代表了电路功能剖析的关键下一阶段。