Functional Dissection of New Retinal Circuits

新视网膜回路的功能解剖

• 批准号: 9188082
• 负责人: Z JIMMY ZHOU
• 金额: $ 41.88万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-12-01 至 2018-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9188082
• 关键词: Address; Amacrine Cells; Calcium; Cell physiology; Complex; Cone; Dendrites; Disease; Dissection; Electrophysiology (science); Feedback; Glutamates; Glycine; Goals; Health; Human; Image; Inner Nuclear Layer; Light; Modeling; Mus; Neurons; Output; Physiological; Physiology; Preparation; Property; Public Health; Publications; Research; Retina; Retinal; Rhodopsin; SLC17A8 gene; Signal Transduction; Site; Synapses; Synaptic Transmission; System; Techniques; Testing; Transgenic Mice; Vision; Visual; base; cell type; connectome; design; experimental study; ganglion cell; insight; neural circuit; neurochemistry; neuronal circuitry; neurotransmission; novel; optogenetics; patch clamp; postsynaptic; postsynaptic neurons; public health relevance; receptive field; relating to nervous system; response; theories; two-photon; visual process

 DESCRIPTION (provided by applicant): The goal of this proposal is to dissect and understand functional neural circuits in the mammalian retina, with a focus on the newly identified glutamatergic amacrine cell (GAC) circuit. The proposed studies are based on our recent finding of unconventional glutamatergic synaptic transmission from GACs to specific ganglion cell types (Lee et al. Neuron, 2014). This finding suggests intriguing neuronal circuits of GACs and new forms of visual computation in the inner retina. Although little is currently known about the GAC circuitry, several distinct advantages of the GAC system in the mouse retina present a rare opportunity for us to investigate (1) how a defined amacrine cell type makes functional connections with its upstream input neurons, (2) how this cell type responds to and processes visual inputs, and (3) how this cell type forms synaptic circuits with downstream target neurons and contributes to visual computation in the inner retina. Being an excitatory (and potentially dual excitatory/inhibitory) amacrine cell type, GACs also provide a unique opportunity for us to investigate the possibility of new forms of co-neurotransmission and test new theories of amacrine cell function in the retina. The proposed studies will address the above questions using a combination of electrophysiology, two-photon imaging, optogenetics, and chemogenetics in a powerful wholemount retinal preparation of a transgenic mouse line in which GACs can be specifically identified and genetically manipulated. The ability to integrate these advanced techniques in a set of carefully designed experiments will allow us to obtain detailed physiological, neurochemical, and circuit information about the GAC network at a level unattainable by other experimental approaches. The proposal will pursue three specific aims: (1) understand the cellular and dendritic response properties of GACs, (2) understand the connectivity and the function of the GAC output circuit, and (3) understand the functional inputs from bipolar cell types to GACs. Results from these studies are expected to provide novel insights into both the connectome and the physiology of a novel retinal circuit and shed important light on retinal circuitry and retinal function in health and disease.