Cellular Imaging Core

细胞成像核心

• 批准号: 9229198
• 负责人: Chinfei Chen
• 金额: $ 9.77万
• 依托单位: BOSTON CHILDREN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-23 至 2021-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9229198
• 关键词: Advisory Committees; Array tomography; Boston; Cells; Computer software; Consultations; Data; Disease; Education; Equipment; Event; Experimental Designs; Fund Raising; Funding; Funding Agency; Future; Goals; Grant; Hour; Image; Image Analysis; Imaging Techniques; In Situ; In Vitro; Intellectual functioning disability; Knowledge; Lasers; Life; Maintenance; Mental Retardation and Developmental Disabilities Research Centers; Microscope; Microscopy; Neurodevelopmental Disorder; Neurology; Neurons; Organ; Organism; Pediatric Hospitals; Research; Research Personnel; Resolution; Sampling; Scanning; Services; Signal Transduction; Site; Staging; Techniques; Technology; Time; Training; United States National Institutes of Health; Vendor; abstracting; awake; cellular imaging; cost; developmental disease; digital imaging; in vivo; instrument; instrumentation; light microscopy; medical schools; member; microscopic imaging; neurodevelopment; new technology; optogenetics; tissue fixing

CELLULAR IMAGING CORE (CORE C) ABSTRACT The research efforts of IDDRC investigators at Boston Children's Hospital and Harvard Medical School have increasingly come to rely on high-end and expensive microscopy and digital imaging approaches. Understanding the underlying mechanisms and structural changes associated with neurodevelopmental disorders require that researchers visualize the morphological and cell signaling events in fixed tissue and in intact network of cells in situ, such as in in vitro organ explants and in vivo awake behaving organisms. The IDDRC Cellular Imaging Core furthers research and advancement of knowledge by lowering barriers to entry for state-of-the-art light microscopy. Our specific aims are: 1) To provide affordable access to high-end microscopy instruments and image analysis workstations. 2) To provide training, consultation and education to IDDRC researchers, and 3) To identify and obtain new equipment and technology that is relevant to the research of IDDRC investigators. The core offers multiphoton, confocal and widefield microscopes as well as image analysis workstations and software that are widely used by IDDRC investigators. Over the past grant period, 32 IDDRC labs have used the core an average of 9,187 hours/year. During this period, the core expanded its capacity and capabilities by raising $1.4 million in outside funding for the purchase of new instrumentation, including a $500,000 NIH shared instrumentation grant for a multiphon/confocal. IDDRC investigators have used the core to investigate and advance our understanding of normal and pathological neural development, plasticity, and function. The goal of the Boston Children's Hospital IDDRC Cellular Imaging Core for this next funding period is to continue to provide IDDRC investigators with affordable access to state-of-the-art equipment, services, training and advice for projects using light microscopy. In fulfilling this goal, the core helps to insure that instrument cost or technical complexity will not slow or thwart research into intellectual disabilities and developmental disorders.

细胞成像核心（核心C） 摘要 波士顿儿童医院和哈佛医学院的IDDRC研究人员的研究成果 越来越依赖于高端和昂贵的显微镜和数字成像方法。 了解与神经发育相关的潜在机制和结构变化 疾病需要研究人员在固定的组织中可视化形态和细胞信号事件 以及原位完整的细胞网络，如体外器官外植体和体内清醒行为 有机体IDDRC细胞成像核心通过以下方式促进研究和知识的进步： 降低了最先进的光学显微镜的准入门槛。我们的具体目标是：1）提供 高端显微镜仪器和图像分析工作站的价格实惠。2)提供 培训，咨询和教育IDDRC研究人员，和3）确定和获得新的 与IDDRC调查人员的研究有关的设备和技术。核心报价 多光子、共焦和宽视场显微镜以及图像分析工作站和软件 被IDDRC调查人员广泛使用。在过去的资助期内，32个IDDRC实验室使用了 核心平均每年9，187小时。在此期间，核心扩大了其容量和能力 通过筹集140万美元的外部资金用于购买新仪器，其中包括50万美元的 NIH共享仪器拨款用于多焦/共焦。IDDRC调查人员使用核心， 研究和推进我们对正常和病理性神经发育，可塑性， 和功能 波士顿儿童医院IDDRC细胞成像核心在下一个资助期的目标是 继续为IDDRC调查人员提供负担得起的最先进的设备， 为使用光学显微镜的项目提供服务、培训和咨询。在实现这一目标的过程中，核心有助于 确保仪器成本或技术复杂性不会减缓或阻碍对知识分子 残疾和发育障碍。