Shared multi-photon confocal microscope

共享多光子共焦显微镜

• 批准号: 8448369
• 负责人: Chinfei Chen
• 金额: $ 49.47万
• 依托单位: BOSTON CHILDREN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-07-15 至 2014-07-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8448369
• 关键词: Applications Grants; Area; Biology; Boston; Cell Proliferation; Cell physiology; Communities; Core Facility; Defect; Funding; Image; Imagery; In Situ; Laboratory Research; Life; Mental Retardation and Developmental Disabilities Research Centers; Microscope; Neurobiology; Neuroglia; Neurology; Neurons; Neurosciences; Pain; Pediatric Hospitals; Process; Request for Applications; Research; Research Personnel; Resolution; Scientist; Signal Transduction; Stem cells; Structure; Synaptic plasticity; System; Systems Development; Technology; Tissues; United States National Institutes of Health; axon guidance; body system; cellular imaging; human disease; instrument; intellectual and developmental disability; medical schools; multi-photon; programs; relating to nervous system; research study; sensory system; tissue fixing

DESCRIPTION (provided by applicant): This grant application requests funding for the purchase of a shared Multiphoton Confocal Microscope System for the Intellectual and Developmental Disabilities Research Center (IDDRC) Imaging Core at Children's Hospital, Boston. This microscope offers state-of-the-art technology that will allow many investigators to visualize fluorescent structures deep into living and fixed tissues at unprecedented spatial resolution and with high sensitivity. These features of the multiphoton confocal system will benefit the growing number of NIH- funded research studies at Children's Hospital and the Harvard Medical School Area that require the imaging of cellular processes deep in live tissue. Research programs that require visualization of fine processes of neuronal networks in situ will also profit strongly from access to this instrument. The modern multiphoton confocal system will be incorporated into the Cellular Imaging Core Facility located in the Department of Neurology and F.M. Kirby Neurobiology Center at Children's Hospital. This facility has been operational for over 34 years and serves more than 74 research laboratories at Children's Hospital and throughout Harvard Medical School. Scientists and clinicians in this research community will be able to use this microscope to study a wide variety of problems in neuroscience and other fields of biology, including how the mechanisms of synaptic plasticity, neural-glia interactions, axon guidance defects, pain, stem cell proliferation, sensory system signaling, and organ system development relate to human disease.

描述（由申请人提供）：本拨款申请要求为波士顿儿童医院的智力和发育障碍研究中心（IDDRC）成像核心购买共享的多光子共聚焦显微镜系统。这种显微镜提供了最先进的技术，将允许许多研究人员可视化荧光结构深入到生活和固定组织在前所未有的空间分辨率和高灵敏度。多光子共聚焦系统的这些特点将有利于越来越多的NIH资助的儿童医院和哈佛医学院地区的研究，这些研究需要对活组织深处的细胞过程进行成像。需要将神经网络精细过程可视化的研究项目也将从使用该仪器中获益良多。现代多光子共聚焦系统将被纳入位于儿童医院神经内科和F.M. Kirby神经生物学中心的细胞成像核心设施。该设施已运行超过34年，为儿童医院和整个哈佛医学院的74多个研究实验室提供服务。该研究社区的科学家和临床医生将能够使用该显微镜研究神经科学和其他生物学领域的各种问题，包括突触可塑性、神经胶质相互作用、轴突引导缺陷、疼痛、干细胞增殖、感觉系统信号和器官系统发育与人类疾病的关系。