Shared multi-photon confocal microscope

描述(由申请人提供)：这份赠款申请申请为波士顿儿童医院的智力和发育障碍研究中心(IDDRC)成像核心申请购买共享的多光子共聚焦显微镜系统。这种显微镜提供了最先进的技术，使许多研究人员能够以前所未有的空间分辨率和高灵敏度可视化深入活组织和固定组织的荧光结构。多光子共聚焦系统的这些功能将使NIH资助的儿童医院和哈佛医学院地区越来越多的研究受益，这些研究需要对活组织深处的细胞过程进行成像。需要原位可视化神经元网络精细过程的研究项目也将从这一仪器的使用中受益匪浅。现代多光子共聚焦系统将被纳入位于儿童医院神经内科和F.M.柯比神经生物学中心的细胞成像核心设施中。该设施已经运营了34年，为儿童医院和整个哈佛医学院的74个研究实验室提供服务。这一研究领域的科学家和临床医生将能够使用这种显微镜来研究神经科学和其他生物学领域的各种问题，包括突触可塑性、神经胶质细胞相互作用、轴突引导缺陷、疼痛、干细胞增殖、感觉系统信号和器官系统发育的机制如何与人类疾病相关。

DESCRIPTION (provided by applicant): This grant application requests funding for the purchase of a shared Multiphoton Confocal Microscope System for the Intellectual and Developmental Disabilities Research Center (IDDRC) Imaging Core at Children's Hospital, Boston. This microscope offers state-of-the-art technology that will allow many investigators to visualize fluorescent structures deep into living and fixed tissues at unprecedented spatial resolution and with high sensitivity. These features of the multiphoton confocal system will benefit the growing number of NIH- funded research studies at Children's Hospital and the Harvard Medical School Area that require the imaging of cellular processes deep in live tissue. Research programs that require visualization of fine processes of neuronal networks in situ will also profit strongly from access to this instrument. The modern multiphoton confocal system will be incorporated into the Cellular Imaging Core Facility located in the Department of Neurology and F.M. Kirby Neurobiology Center at Children's Hospital. This facility has been operational for over 34 years and serves more than 74 research laboratories at Children's Hospital and throughout Harvard Medical School. Scientists and clinicians in this research community will be able to use this microscope to study a wide variety of problems in neuroscience and other fields of biology, including how the mechanisms of synaptic plasticity, neural-glia interactions, axon guidance defects, pain, stem cell proliferation, sensory system signaling, and organ system development relate to human disease.