Shared High-resolution Laser Scanning Microscope with Airyscan 2

摘要 该补助金申请要求提供资金支持，用于购买共享共焦显微镜系统， 一个高分辨率的探测器将被安装在智力和发育障碍研究中心 Cellular Imaging Core是波士顿儿童医院（BCH）的一个共享显微镜设施。请求的 仪器结合了高信噪比、快速采集速度和增强的 这是该领域其他仪器无法实现的。这些功能将使A 越来越多的NIH资助的研究在BCH和哈佛医学院，需要亚细胞 以高采集速度在固定和活组织中定位蛋白质。其他研究项目需要 翻译应用的高分辨率和高通量筛选将从中受益匪浅 仪器 安装在神经生物学系和F.M. Kirby神经生物学 中心在BCH，这种国家的最先进的显微镜将有利于84哈佛医学院地区实验室 使用核心设施进行基础和转化研究。这些实验室的主要研究者将使用 系统研究各种各样的紧迫问题，有关细胞器的流动性和动力学，亚细胞 结构，蛋白质-蛋白质和细胞-细胞相互作用，以及这些基本过程在正常情况下的关系 脑发育，轴突再生和神经元存活，以及神经发育和 神经退行性疾病

ABSTRACT This grant application requests funding support for the purchase of a shared Confocal Microscope System with a high-resolution detector to be installed in the Intellectual and Developmental Disabilities Research Center Cellular Imaging Core, a shared microscopy facility at Boston Children’s Hospital (BCH). The requested instrument combines enhancements in high signal-to-noise ratio, fast acquisition speed and enhanced resolution that are not achievable by other instruments available in the area. These features will benefit a growing number of NIH-funded research studies at BCH and Harvard Medical School that require sub-cellular localization of proteins in fixed and living tissue at high acquisition speeds. Other research programs that need high-resolution & high-throughput screening for translational applications will benefit immensely from this instrument. After installation in the Cellular Imaging Core in the Department of Neurobiology and F.M. Kirby Neurobiology Center at BCH, this state-of-the-art microscope will benefit the 84 Harvard Medical School area laboratories that use the core facility for basic and translational research. Primary Investigators from these labs will use the system to study a wide variety of pressing questions regarding organelle mobility and dynamics, subcellular architecture, protein-protein and cell-cell interactions, and the relations of these basic processes in normal brain development, in axon regeneration and neuronal survival, and in neurodevelopmental and neurodegenerative disorders.