Shared High-resolution Laser Scanning Microscope with Airyscan 2

摘要 此赠款申请申请为购买共享共焦显微镜系统提供资金支持 将在智力和发育障碍研究中心安装高分辨率探测器 细胞成像核心，波士顿儿童医院(BCH)的一个共享显微镜设备。所请求的 仪器结合了高信噪比、快速采集速度和增强 这些决议是该地区现有的其他文书无法实现的。这些功能将使 BCH和哈佛医学院越来越多的NIH资助的研究需要亚细胞 蛋白质在固定的和活的组织中以高速采集的定位。其他需要的研究项目 翻译应用程序的高分辨率和高通量筛选将从中受益匪浅 乐器。 在神经生物学系和F.M.Kirby神经生物学系的细胞成像核心安装后 这一最先进的显微镜将使84个哈佛医学院地区实验室受益 使用核心设施进行基础研究和翻译研究。来自这些实验室的主要调查人员将使用 研究细胞器运动和动力学的各种紧迫问题的系统，亚细胞 结构、蛋白质-蛋白质和细胞-细胞相互作用以及这些基本过程在正常情况下的关系 大脑发育，轴突再生和神经元存活，以及神经发育和 神经退行性疾病。

ABSTRACT This grant application requests funding support for the purchase of a shared Confocal Microscope System with a high-resolution detector to be installed in the Intellectual and Developmental Disabilities Research Center Cellular Imaging Core, a shared microscopy facility at Boston Children’s Hospital (BCH). The requested instrument combines enhancements in high signal-to-noise ratio, fast acquisition speed and enhanced resolution that are not achievable by other instruments available in the area. These features will benefit a growing number of NIH-funded research studies at BCH and Harvard Medical School that require sub-cellular localization of proteins in fixed and living tissue at high acquisition speeds. Other research programs that need high-resolution & high-throughput screening for translational applications will benefit immensely from this instrument. After installation in the Cellular Imaging Core in the Department of Neurobiology and F.M. Kirby Neurobiology Center at BCH, this state-of-the-art microscope will benefit the 84 Harvard Medical School area laboratories that use the core facility for basic and translational research. Primary Investigators from these labs will use the system to study a wide variety of pressing questions regarding organelle mobility and dynamics, subcellular architecture, protein-protein and cell-cell interactions, and the relations of these basic processes in normal brain development, in axon regeneration and neuronal survival, and in neurodevelopmental and neurodegenerative disorders.