Shared High-resolution Laser Scanning Microscope with Airyscan 2

摘要 该拨款申请请求资金支持，以购买共享的共焦显微镜系统 将安装在智力和发育障碍研究中心的高分辨率探测器 Cellular Imaging Core，波士顿儿童医院 (BCH) 的共享显微镜设施。所要求的 仪器结合了高信噪比、快速采集速度和增强的增强功能 该分辨率是该领域其他可用工具无法实现的。这些功能将有利于 BCH 和哈佛医学院越来越多的 NIH 资助的研究需要亚细胞 以高采集速度定位固定组织和活组织中的蛋白质。其他需要的研究项目 用于转化应用的高分辨率和高通量筛选将从中受益匪浅 仪器。 安装在神经生物学和调频系的细胞成像核心后柯比神经生物学 BCH 中心的这款最先进的显微镜将使哈佛医学院的 84 个地区实验室受益 使用核心设施进行基础和转化研究。这些实验室的主要研究人员将使用 系统研究有关细胞器流动性和动力学、亚细胞的各种紧迫问题 结构、蛋白质-蛋白质和细胞-细胞相互作用，以及正常情况下这些基本过程的关系 大脑发育、轴突再生和神经元存活以及神经发育和 神经退行性疾病。

ABSTRACT This grant application requests funding support for the purchase of a shared Confocal Microscope System with a high-resolution detector to be installed in the Intellectual and Developmental Disabilities Research Center Cellular Imaging Core, a shared microscopy facility at Boston Children’s Hospital (BCH). The requested instrument combines enhancements in high signal-to-noise ratio, fast acquisition speed and enhanced resolution that are not achievable by other instruments available in the area. These features will benefit a growing number of NIH-funded research studies at BCH and Harvard Medical School that require sub-cellular localization of proteins in fixed and living tissue at high acquisition speeds. Other research programs that need high-resolution & high-throughput screening for translational applications will benefit immensely from this instrument. After installation in the Cellular Imaging Core in the Department of Neurobiology and F.M. Kirby Neurobiology Center at BCH, this state-of-the-art microscope will benefit the 84 Harvard Medical School area laboratories that use the core facility for basic and translational research. Primary Investigators from these labs will use the system to study a wide variety of pressing questions regarding organelle mobility and dynamics, subcellular architecture, protein-protein and cell-cell interactions, and the relations of these basic processes in normal brain development, in axon regeneration and neuronal survival, and in neurodevelopmental and neurodegenerative disorders.