Dynamin-related protein 1, neurodegeneration and Huntington's disease

动力相关蛋白 1、神经变性和亨廷顿病

• 批准号: 9285853
• 负责人: XIN QI
• 金额: $ 34.67万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-06-01 至 2019-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9285853
• 关键词: ATP phosphohydrolase; Animal Disease Models; Animals; Basic Science; Behavior; Behavioral; Biochemical; Biological Models; Brain; Cell Culture Techniques; Cell Death; Cells; Chronic; Clinical Research; Code; Complex; Corpus striatum structure; DNA Sequence Alteration; Data; Development; Disease; Dynamin; Exons; Family; Genes; Glutamine; Goals; Guanosine Triphosphate Phosphohydrolases; Human; Huntington Disease; Huntington gene; Image; Impairment; Length; MAPK1 gene; Mediating; Mitochondria; Modeling; Molecular; Morphology; Mus; Nerve Degeneration; Neurites; Neurodegenerative Disorders; Neurologic; Neurologic Symptoms; Neurons; Pathogenesis; Pathology; Patients; Peptides; Pharmacology; Play; Process; Protein-Serine-Threonine Kinases; Proteins; Proteomics; Reagent; Role; Signal Transduction; Symptoms; Therapeutic; Transgenic Mice; Treatment Efficacy; Tubular formation; Work; design; effective therapy; in vitro Model; in vivo; induced pluripotent stem cell; inhibitor/antagonist; member; mitochondrial dysfunction; motor deficit; mouse model; mutant; neuron loss; neuropathology; neurotoxicity; novel; novel strategies; novel therapeutic intervention; novel therapeutics; protective effect; protein protein interaction; public health relevance; research study; tool

DESCRIPTION (provided by applicant): Huntington's disease (HD) is a fatal, autosomal dominant, neurodegenerative disorder caused by a glutamine-coding CAG expansion within exon 1 of the huntingtin gene. Although the genetic mutation associated with the disease has been identified, the molecular and cellular basis of HD is not yet understood and successful treatment for this disease remains elusive. Basic research and clinical studies indicate that mitochondrial dysfunction plays an important role in the pathogenesis of HD. Mitochondria are organized in a highly dynamic tubular network that is continuously reshaped by opposing processes of fusion and fission. Dynamin-related protein 1 (Drp1) is a large GTPase and a key protein governing mitochondrial fission. Recent studies have highlighted the causal role of Drp1-mediated excessive mitochondrial fission in neuronal death in HD cell culture models. However, how Drp1 hyperactivation mediates mitochondrial damage and neurodegeneration in HD and whether pharmacological inhibition of Drp1 activation is sufficient to reduce mutant Htt (mtHtt)-induced neurotoxicity and neurodegeneration are not known. Our recent work showed that Drp1 is translocated to the mitochondria and hyper-activated in both HD cell cultures and in vivo in the HD R6/2 transgenic mouse brain. Importantly, using a novel and selective peptide inhibitor of Drp1, P110, recently developed in our group, we found that inhibition of Drp1-dependent mitochondrial impairment corrected mitochondrial dysfunction and neuronal cell death in HD cell cultures, and reduced behavioral deficits and loss of striatal neurons in HD R6/2 transgenic mice. Moreover, treatment with P110 corrected mitochondrial morphology and reduced neurite loss and cell death in GABAergic striatal neurons derived from HD patient- induced pluripotent stem cells (HD-iPS cells). Further, using unbiased proteomic analysis, we recently profiled the interactome of Drp1 in neuronal cultures derived from HD patient-iPS cells. Our preliminary studies identified two mechanistically distinct candidate proteins (ATADA3, a member of mitochondrial AAA-ATPase family, and MAPK1, a serine/threonine kinase) that are involved in Drp1-mediated neuronal damage. These lines of evidence indicate that Drp1 hyperactivation is a predominant cause of neurodegeneration in HD. Thus, we hypothesize that inhibition of Drp1-mediated mitochondrial damage is a novel approach for reducing neuropathology in HD models in vitro and in vivo. Using biochemical, imaging, bio-energetic, proteomic and pharmacological approaches ranging from animals to patient neurons, our goal in this application is to unravel the complexity of Drp1-mediated mitochondrial dysfunction in neurodegeneration in both mechanistic and therapeutic detail. The proposed study will produce novel information on the role of Drp1-mediated mitochondrial fission in the pathogenesis of HD and provide a useful model system in which to study mitochondrial pathology in striatal neurons. We will also generate pharmacological tools to inhibit HD pathogenesis as a first step towards the development of novel therapeutics for HD.

描述（由申请方提供）：亨廷顿病（HD）是一种致死性常染色体显性遗传神经退行性疾病，由亨廷顿基因外显子1内的谷氨酰胺编码CAG扩增引起。虽然与该疾病相关的基因突变已被确定，但HD的分子和细胞基础尚未被理解，并且该疾病的成功治疗仍然难以捉摸。基础研究和临床研究表明，线粒体功能障碍在HD的发病机制中起重要作用。线粒体组织在一个高度动态的管状网络中，通过融合和分裂的相反过程不断重塑。动力蛋白相关蛋白1（Dynamin-related protein 1，Drp 1）是一个大的GT3，是控制线粒体分裂的关键蛋白。最近的研究强调了在HD细胞培养模型中Drp 1介导的过度线粒体分裂在神经元死亡中的因果作用。然而，目前尚不清楚Drp 1过度激活如何介导HD中的线粒体损伤和神经退行性变，以及Drp 1激活的药理学抑制是否足以减少突变型Htt（mtHtt）诱导的神经毒性和神经退行性变。我们最近的工作表明，Drp 1易位到线粒体和超激活的HD细胞培养物和体内的HD R6/2转基因小鼠大脑。重要的是，使用一种新的和选择性的肽抑制剂Drp 1，P110，最近在我们的小组，我们发现，抑制Drp 1依赖性线粒体损伤纠正线粒体功能障碍和神经元细胞死亡在HD细胞培养，并减少行为缺陷和损失的纹状体神经元在HD R6/2转基因小鼠。此外，用P110处理校正了线粒体形态并减少了源自HD患者诱导的多能干细胞（HD-iPS细胞）的GABA能纹状体神经元中的神经突损失和细胞死亡。此外，使用公正的蛋白质组学分析，我们最近描绘了来自HD患者iPS细胞的神经元培养物中Drp 1的相互作用组。我们的初步研究确定了两个机制不同的候选蛋白（ATADA 3，线粒体AAA-ATP酶家族的成员，和MAPK 1，丝氨酸/苏氨酸激酶），参与Drp 1介导的神经元损伤。这些证据表明，Drp 1过度激活是HD神经变性的主要原因。因此，我们假设抑制Drp 1介导的线粒体损伤是一种新的方法，在体外和体内减少HD模型的神经病理学。使用从动物到患者神经元的生物化学，成像，生物能量，蛋白质组学和药理学方法，我们在本申请中的目标是在机制和治疗细节上揭示神经变性中Drp 1介导的线粒体功能障碍的复杂性。拟议的研究将产生新的信息Drp 1介导的线粒体分裂在HD的发病机制中的作用，并提供了一个有用的模型系统，在其中研究纹状体神经元的线粒体病理。我们还将产生抑制HD发病机制的药理学工具，作为开发HD新疗法的第一步。