GENETIC, IMMUNOLOGIC AND MECHANISTIC BASIS OF HUMAN NK CELL DEFICIENCY
人类 NK 细胞缺陷的遗传、免疫学和机制基础
基本信息
- 批准号:9205454
- 负责人:
- 金额:$ 71.48万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2016
- 资助国家:美国
- 起止时间:2016-01-19 至 2020-12-31
- 项目状态:已结题
- 来源:
- 关键词:AffectBasic ScienceBiologicalBiological AssayBiological ModelsBiologyBypassCD34 geneCRISPR/Cas technologyCandidate Disease GeneCell CountCell Differentiation processCell LineCell physiologyCellsCellular biologyChildClinicalCollaborationsDataDefectDevelopmentDiagnosticDiseaseEtiologyEvaluationFlow CytometryGenesGeneticGenetic studyGoldGuidelinesHealthHumanImageImmuneImmune systemImmunologicsImmunologistIn VitroIndividualInflammatoryJournalsKnock-outLaboratoriesLyticMCM10 geneMalignant NeoplasmsMedicineMutationNatural Killer CellsPatientsPhenotypePredispositionProcessPublishingRecruitment ActivityResolutionRoleSamplingStem cellsSynapsesTechniquesTherapeuticTimeValidationViral CancerVirus DiseasesWestern BlottingWorkbasecohortcollegecongenital immunodeficiencycytokinedesignexome sequencingexperimental studyfield studyhumanized mouseimmunological synapseimmunoregulationin vivo Modelinduced pluripotent stem cellinnovationinsightkillingsknock-downknockout genenovelprogramspublic health relevancereceptorreconstitutionsegregationsmall hairpin RNAtooltranscription factorwhole genome
项目摘要
DESCRIPTION (provided by applicant): Natural Killer (NK) cell deficiency (NKD) is a recently appreciated subset of primary immunodeficiency (PID) in which NK cell deficits represent the main immunological defect. These diseases are typically severe; patients suffer from profound and often lethal susceptibility to viral infection and cancer. To date, only three NKD genes have been identified and little is known of the biological mechanisms by which they interfere with NK cell numbers and/or function. At the same time, no good therapeutic options are available for those affected. For over a decade, our laboratory has sought genetic, immunologic, and therapeutic solutions to these rare, but deadly diseases. In the process, we have established a robust worldwide referral base, assembled an expert team of geneticists, immunologists and clinicians, and are now poised to dramatically advance our understanding of NKD and the role of NK cells in health and disease. Through two Aims, we will define genes responsible for human NKD (Aim 1), and determine their relevance through intensive biological characterization (Aim 2). In Aim 1, we use whole exome sequencing and tandem chromosomal microarray to identify NKD mutations in the world's largest cohort of NKD patients. We have already identified 7 novel gene candidates using this approach. In Aim 2, we will rigorously evaluate our candidate NKD genes, defining their roles in NK cell cytolytic functions, developmental maturation, receptor repertoire, and immunoregulatory and inflammatory function using a carefully thought out progression of cutting-edge immunologic experiments. Tools to be used include "gold-standard" 51Cr-release killing assays, Western blots, shRNA knockdown, gene knockout using CRISPR/Cas technology, extended multiparameter flow cytometry-based phenotypic and functional analysis, high- and super-resolution quantification of the lytic immunological synapse, imaging flow cytometry quantification of transcription factor activation and lytic synapse dynamics, and in vitro NK cell differentiation from CD34+ hematopoetic stem cells. Specifically, we will prove that these genes cause NKD in ex vivo NKD patient cells, ex vivo healthy donor NK cells and NK cell lines ("knock-outs" and "knock-ins"), and patient-derived induced pluripotent stem cells (iPSC) and reconstituted humanized mice. Additionally, the potential therapeutic benefit of clinically available cytokines will be evaluated for each candidate. Overall our approach represents iterative cycles of genetic discovery, confirmation, biological validation,
and immunologic insight. We are confident that these innovative approaches, combined with our established NK cell and genetic expertise, and privileged NKD patient pipeline, will drive meaningful diagnostic, therapeutic, and basic science progress in this burgeoning field. We provide preliminary data from two examples of our discoveries, ATP6V0A2 and MCM10, as concrete examples of how the proposed work will substantively advance the field.
描述(由申请人提供):自然杀伤(NK)细胞缺乏症(NKD)是最近认识到的原发性免疫缺陷(PID)的一个子集,其中NK细胞缺陷代表主要的免疫缺陷。这些疾病通常是严重的;患者对病毒感染和癌症具有深刻且往往致命的易感性。到目前为止,只有三个NKD基因已被确定,很少有人知道的生物学机制,他们干扰NK细胞的数量和/或功能。与此同时,受影响的人没有良好的治疗选择。十多年来,我们的实验室一直在寻找这些罕见但致命的疾病的遗传,免疫和治疗解决方案。在这个过程中,我们建立了一个强大的全球转诊基础,组建了一个由遗传学家、免疫学家和临床医生组成的专家团队,现在我们正准备大大提高我们对NKD以及NK细胞在健康和疾病中的作用的理解。通过两个目标,我们将定义负责人类NKD的基因(目标1),并通过深入的生物学表征确定它们的相关性(目标2)。在目标1中,我们使用全外显子组测序和串联染色体微阵列来鉴定世界上最大的NKD患者队列中的NKD突变。我们已经使用这种方法鉴定了7个新的候选基因。在目标2中,我们将严格评估我们的候选NKD基因,使用经过深思熟虑的尖端免疫学实验进展来定义它们在NK细胞溶细胞功能、发育成熟、受体库以及免疫调节和炎症功能中的作用。待使用的工具包括“金标准”51 Cr释放杀伤测定、蛋白质印迹、shRNA敲除、使用CRISPR/Cas技术的基因敲除、基于扩展多参数流式细胞术的表型和功能分析、裂解免疫突触的高分辨率和超分辨率定量、转录因子激活和裂解突触动力学的成像流式细胞术定量、以及从CD 34+造血干细胞体外分化NK细胞。具体而言,我们将证明这些基因在离体NKD患者细胞、离体健康供体NK细胞和NK细胞系(“敲除”和“敲入”)以及患者来源的诱导多能干细胞(iPSC)和重建的人源化小鼠中引起NKD。此外,将对每种候选细胞因子的临床可用细胞因子的潜在治疗获益进行评价。总的来说,我们的方法代表了遗传发现、确认、生物学验证的迭代周期,
和免疫学洞察力。我们相信,这些创新方法,结合我们成熟的NK细胞和遗传专业知识,以及NKD患者的特权管道,将推动这一新兴领域有意义的诊断,治疗和基础科学进步。我们提供了我们发现的两个例子ATP 6V 0A 2和MCM 10的初步数据,作为拟议工作将如何实质性推进该领域的具体例子。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
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Jordan Scott Orange其他文献
Jordan Scott Orange的其他文献
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{{ truncateString('Jordan Scott Orange', 18)}}的其他基金
GENETIC, IMMUNOLOGIC AND MECHANISTIC BASIS OF HUMAN NK CELL DEFICIENCY
人类 NK 细胞缺陷的遗传、免疫学和机制基础
- 批准号:
10363767 - 财政年份:2016
- 资助金额:
$ 71.48万 - 项目类别:
GENETIC, IMMUNOLOGIC AND MECHANISTIC BASIS OF HUMAN NK CELL DEFICIENCY
人类 NK 细胞缺陷的遗传、免疫学和机制基础
- 批准号:
10490860 - 财政年份:2016
- 资助金额:
$ 71.48万 - 项目类别:
GENETIC, IMMUNOLOGIC AND MECHANISTIC BASIS OF HUMAN NK CELL DEFICIENCY
人类 NK 细胞缺陷的遗传、免疫学和机制基础
- 批准号:
10686199 - 财政年份:2016
- 资助金额:
$ 71.48万 - 项目类别:
GENETIC, IMMUNOLOGIC AND MECHANISTIC BASIS OF HUMAN NK CELL DEFICIENCY
人类 NK 细胞缺陷的遗传、免疫学和机制基础
- 批准号:
9003675 - 财政年份:2016
- 资助金额:
$ 71.48万 - 项目类别:
Directing Function at the Natural Killer Cell Secretory Immunological Synapse
自然杀伤细胞分泌免疫突触的指导功能
- 批准号:
8308767 - 财政年份:2011
- 资助金额:
$ 71.48万 - 项目类别:
Directing Function at the Natural Killer Cell Secretory Immunological Synapse
自然杀伤细胞分泌免疫突触的指导功能
- 批准号:
7875101 - 财政年份:2009
- 资助金额:
$ 71.48万 - 项目类别:
The mechanism of NK cell defects in human NEMO deficiency
人类NEMO缺陷导致NK细胞缺陷的机制
- 批准号:
7629124 - 财政年份:2008
- 资助金额:
$ 71.48万 - 项目类别:
The mechanism of NK cell defects in human NEMO deficiency
人类NEMO缺陷导致NK细胞缺陷的机制
- 批准号:
7530223 - 财政年份:2008
- 资助金额:
$ 71.48万 - 项目类别:
Directing Function at the Natural Killer Cell Secretory Immunological Synapse
自然杀伤细胞分泌免疫突触的指导功能
- 批准号:
7650556 - 财政年份:2008
- 资助金额:
$ 71.48万 - 项目类别:
Directing Function at the Natural Killer Cell Secretory Immunological Synapse
自然杀伤细胞分泌免疫突触的指导功能
- 批准号:
8448575 - 财政年份:2007
- 资助金额:
$ 71.48万 - 项目类别:
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