Concurrent multiphoton microscopy and magnetic resonance imaging (COMPMRI)

并行多光子显微镜和磁共振成像 (COMPMRI)

• 批准号: 9360610
• 负责人: Yi Wang
• 金额: $ 18.74万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-30 至 2019-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9360610
• 关键词: Area; BRAIN initiative; Behavior; Biomedical Research; Brain; Brain region; Brain scan; Budgets; Cells; Craniotomy; Development; Environment; Epilepsy; Event; Fluorescence; Functional Magnetic Resonance Imaging; Genetic; Genetic Engineering; Head; Histology; Human; Image; Individual; Knowledge; Lasers; Link; Magnetic Resonance Imaging; Measures; Methodological Studies; Microscopy; Molecular; Mus; Myelin; Nervous system structure; Neurons; Optics; Pattern; Penetration; Photons; Preparation; RF coil; Research; Resolution; Seizures; Signal Transduction; Testing; Therapeutic; Thinness; Variant; base; biological research; cellular imaging; cranium; experience; high resolution imaging; imaging study; imaging system; multiphoton imaging; neural circuit; neuronal circuitry; programs; relating to nervous system; response; tool; two-photon

We propose a concurrent multiphoton magnetic resonance imaging (COMPMRI) system for mouse imaging, in response to RFA-EY-16-001, BRAIN Initiative: New Concepts and Early-Stage Research for Large-Scale Recording and Modulation in the Nervous System (R21). We synergistically combine multiphoton microscopy (MPM), which offers cellular imaging resolution with penetration depth, and Magnetic resonance imaging (MRI), which is routinely used to capture large-scale neural activities across many regions of the brain. To understand molecular cellular origin of the large-scale neural activity, we need simultaneous imaging both at cellular resolution and over the whole brain, which is concurrent MPM and MRI as in our proposed COMPMRI. Furthermore, we will leverage our latest breakthrough in MPM using 3-photon excitation with long wavelength that enables through-skull cellular resolution imaging, making it possible to perform MPM at multiple cortical regions. Our proposed COMPMRI will take advantage of this 3-photon microscopy and the large bore size of a 3T human MRI system to scan the brain of a live mouse. Our proposed COMPMRI system will allow simultaneous study of neural activities localized in a region and distributed over the whole brain.

我们提出了一个并发的多光子磁共振成像（COMPMRI）用于鼠标成像的系统， 对RFA-EY-16-001的反应，大脑倡议：大规模的新概念和早期研究 神经系统中的记录和调节（R21）。我们协同结合多光子显微镜 （MPM），它提供具有穿透深度和磁共振成像的细胞成像分辨率 （MRI），通常用于捕获大脑许多地区的大规模神经活动。到 了解大规模神经活动的分子细胞来源，我们需要同时对 细胞分辨率和整个大脑，如我们所提出的CompMRI中，这是同时发生的MPM和MRI。 此外，我们将使用长波长的3光量激发来利用我们在MPM中的最新突破 这样可以通过Skull细胞分辨率成像，使得可以在多个皮质上执行MPM 地区。我们提出的compmri将利用这个3光子显微镜和A的大孔的大小 3T人类MRI系统扫描活鼠的大脑。我们提议的CompMRI系统将允许 同时研究位于一个地区并分布在整个大脑中的神经活动。