Concurrent multiphoton microscopy and magnetic resonance imaging (COMPMRI)

我们提出了一种用于小鼠成像的并行多光子磁共振成像（COMPMRI）系统， 对RFA-EY-16-001《大脑倡议：大规模研究的新概念和早期研究》的答复 神经系统的记录和调制（R21）。我们协同联合收割机多光子显微镜 (MPM)，它提供了细胞成像分辨率与穿透深度，和磁共振成像 (MRI)，它通常用于捕获大脑许多区域的大规模神经活动。到 为了了解大规模神经活动的分子细胞起源，我们需要同时成像， 细胞分辨率和整个大脑，这是并行的MPM和MRI，如在我们提出的COMPMRI。 此外，我们将利用我们在MPM中的最新突破，使用长波长的3光子激发 这使得通过颅骨细胞分辨率成像，使其有可能在多个皮层进行MPM 地区我们提出的COMPMRI将利用这种3光子显微镜和大口径的 3 T人类MRI系统扫描活老鼠的大脑。我们提出的COMPMRI系统将允许 同时研究局部神经活动和分布在整个大脑的神经活动。

We propose a concurrent multiphoton magnetic resonance imaging (COMPMRI) system for mouse imaging, in response to RFA-EY-16-001, BRAIN Initiative: New Concepts and Early-Stage Research for Large-Scale Recording and Modulation in the Nervous System (R21). We synergistically combine multiphoton microscopy (MPM), which offers cellular imaging resolution with penetration depth, and Magnetic resonance imaging (MRI), which is routinely used to capture large-scale neural activities across many regions of the brain. To understand molecular cellular origin of the large-scale neural activity, we need simultaneous imaging both at cellular resolution and over the whole brain, which is concurrent MPM and MRI as in our proposed COMPMRI. Furthermore, we will leverage our latest breakthrough in MPM using 3-photon excitation with long wavelength that enables through-skull cellular resolution imaging, making it possible to perform MPM at multiple cortical regions. Our proposed COMPMRI will take advantage of this 3-photon microscopy and the large bore size of a 3T human MRI system to scan the brain of a live mouse. Our proposed COMPMRI system will allow simultaneous study of neural activities localized in a region and distributed over the whole brain.