Concurrent multiphoton microscopy and magnetic resonance imaging (COMPMRI)

我们提出了一种用于小鼠成像的并行多光子磁共振成像(COMPMRI)系统，在 对RFA-EY-16-001的响应，大脑倡议：大规模的新概念和早期研究 神经系统的记录和调制(R21)。我们协同结合了多光子显微镜 (MPM)，它提供穿透深度的细胞成像分辨率，以及磁共振成像 (核磁共振)，它通常被用来捕捉大脑许多区域的大规模神经活动。至 了解大规模神经活动的分子细胞起源，我们需要同时成像在 细胞分辨率和整个大脑，这是并行的MPM和MRI，就像我们建议的COMPMRI。 此外，我们将利用我们在MPM方面的最新突破，使用长波长的三光子激发 这使穿透颅骨的细胞分辨率成像成为可能，使在多个皮质进行MPM成为可能 地区。我们建议的COMPMRI将利用这种3光子显微镜和大口径的 3T人体核磁共振系统扫描活体小鼠的脑。我们建议的COMPMRI系统将允许 同时研究局部区域和分布在整个大脑中的神经活动。

We propose a concurrent multiphoton magnetic resonance imaging (COMPMRI) system for mouse imaging, in response to RFA-EY-16-001, BRAIN Initiative: New Concepts and Early-Stage Research for Large-Scale Recording and Modulation in the Nervous System (R21). We synergistically combine multiphoton microscopy (MPM), which offers cellular imaging resolution with penetration depth, and Magnetic resonance imaging (MRI), which is routinely used to capture large-scale neural activities across many regions of the brain. To understand molecular cellular origin of the large-scale neural activity, we need simultaneous imaging both at cellular resolution and over the whole brain, which is concurrent MPM and MRI as in our proposed COMPMRI. Furthermore, we will leverage our latest breakthrough in MPM using 3-photon excitation with long wavelength that enables through-skull cellular resolution imaging, making it possible to perform MPM at multiple cortical regions. Our proposed COMPMRI will take advantage of this 3-photon microscopy and the large bore size of a 3T human MRI system to scan the brain of a live mouse. Our proposed COMPMRI system will allow simultaneous study of neural activities localized in a region and distributed over the whole brain.