Generating new mouse mutant strains

产生新的小鼠突变品系

• 批准号: 9790852
• 负责人: Karl Eric Pfeifer
• 金额: $ 62.49万
• 依托单位: EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9790852
• 关键词: Bone Matrix; CRISPR/Cas technology; Communities; Cryopreservation; Disease; ES Cell Line; Embryo; Enhancers; Epigenetic Process; Extracellular Matrix; Functional disorder; Gene Expression Regulation; Genetic Diseases; Genome Stability; Goals; Hematopoiesis; Immune system; Injections; Intramural Research Program; Iron; Malaria; Metabolism; Modeling; Mouse Strains; Mus; Mutant Strains Mice; Mutate; National Institute of Child Health and Human Development; Nuclear; Pathway interactions; Protocols documentation; RNA; Research; Research Personnel; Ribonuclease H; Risk; Scientist; Services; Signal Pathway; T-Cell Development; Transfection; United States National Institutes of Health; Work; blastocyst; cancer genetics; design; embryonic stem cell; malignant endocrine gland neoplasm; mouse model; nervous system development; novel; stem cell biology; zygote

During the last year our research core has worked with ten NIH research units to generate novel embryonic stem cell lines (24 projects, most of them using CRISPR/Cas9 transfections) and new mouse models (36 projects, including 6 CRISPR/Cas9 Zygote injections). This research included establishment of models to study disease associated with RNAse H dysfunction, signal pathway interactions in the immune system, T cell development, hematopoiesis, stem cell biology, epigenetic inheritance in the early embryo, mechanisms regulating genomic stability, RNA metabolism, genetic disorders in bone and extracellular matrix, gene regulation and enhancer function, nervous system development, iron methabolism and malaria risk, and endocrine and cancer genetics. We also performed cryopreservation of 4 mouse lines, derived 14 novel embryonic stem cell lines from genetically altered mice, and re-derived 4 mouse strains. In the past year we developed further expertise to perform zygote injections (nuclear and cytoplasmic) and offered these services to the NICHD research community.

在过去的一年里，我们的研究核心与10个NIH研究单位合作，产生了新的胚胎干细胞系（24个项目，其中大部分使用CRISPR/Cas9转染）和新的小鼠模型（36个项目，包括6个CRISPR/Cas9 Zygote注射）。 这项研究包括建立模型，以研究与RNA酶H功能障碍相关的疾病，免疫系统中的信号通路相互作用，T细胞发育，造血，干细胞生物学，早期胚胎中的表观遗传遗传，调节基因组稳定性的机制，RNA代谢，骨和细胞外基质中的遗传疾病，基因调节和增强子功能，神经系统发育，蛋氨酸铁和疟疾风险，以及内分泌和癌症遗传学。我们还对4个小鼠品系进行了冷冻保存，从基因改变的小鼠中获得了14个新的胚胎干细胞系，并重新获得了4个小鼠品系。 在过去的一年中，我们开发了进一步的专业知识来执行受精卵注射（细胞核和细胞质），并为NICHD研究社区提供这些服务。