Generating new mouse mutant strains

在过去的一年里，我们的研究核心与NIH的十个研究单位合作，产生了新的胚胎干细胞系（24个项目，其中大部分使用CRISPR/Cas9转染）和新的小鼠模型（36个项目，其中包括6个CRISPR/Cas9 Zygote注射）。本研究包括建立与RNAse H功能障碍相关疾病的模型、免疫系统信号通路相互作用、T细胞发育、造血、干细胞生物学、早期胚胎表观遗传、基因组稳定性调节机制、RNA代谢、骨和细胞外基质遗传疾病、基因调控和增强子功能、神经系统发育、铁代谢和疟疾风险、内分泌和癌症遗传学。我们还对4个小鼠品系进行了冷冻保存，从转基因小鼠中获得了14个新的胚胎干细胞品系，并重新获得了4个小鼠品系。

During the last year our research core has worked with ten NIH research units to generate novel embryonic stem cell lines (24 projects, most of them using CRISPR/Cas9 transfections) and new mouse models (36 projects, including 6 CRISPR/Cas9 Zygote injections). This research included establishment of models to study disease associated with RNAse H dysfunction, signal pathway interactions in the immune system, T cell development, hematopoiesis, stem cell biology, epigenetic inheritance in the early embryo, mechanisms regulating genomic stability, RNA metabolism, genetic disorders in bone and extracellular matrix, gene regulation and enhancer function, nervous system development, iron methabolism and malaria risk, and endocrine and cancer genetics. We also performed cryopreservation of 4 mouse lines, derived 14 novel embryonic stem cell lines from genetically altered mice, and re-derived 4 mouse strains. In the past year we developed further expertise to perform zygote injections (nuclear and cytoplasmic) and offered these services to the NICHD research community.