Genomics

基因组学

• 批准号: 9406258
• 负责人: CRAIG B THOMPSON
• 金额: $ 73.44万
• 依托单位: SLOAN-KETTERING INST CAN RESEARCH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9406258
• 关键词: Base Sequence; Basic Science; Bioinformatics; Cancer Center Support Grant; Chromosome Structures; Clinical Research; DNA; DNA analysis; Data; Data Analyses; Detection; Dideoxy Chain Termination DNA Sequencing; Experimental Designs; Gene Chips; Gene Expression; Genome Data Analysis Center; Genomics; Glioblastoma; Grant; Institution; Life; Lung Adenocarcinoma; Measurement; Measures; Memorial Sloan-Kettering Cancer Center; Methylation; MicroRNAs; Microarray Analysis; Mission; Ovarian Serous Cystadenocarcinoma; Performance; Phase; Preparation; Publications; Quality Control; RNA; RNA analysis; Research; Research Personnel; Research Support; Sampling; Scientist; Services; Source; Spottings; Technology; The Cancer Genome Atlas; Time; Training; Training Support; Translational Research; Validation; Variant; Work; anticancer research; cancer genome; chromatin immunoprecipitation; comparative genomic hybridization; epigenomics; functional genomics; genome-wide; high throughput analysis; interest; next generation sequencing; programs; sequencing platform; transcriptome sequencing

The Genomics Core Lab (GCL) is a full-service microarray analysis and sequencing facility. The Core supports basic, translational and clinical research by providing DNA and RNA analysis services from experimental design and sample preparation to basic data analysis and training. Sample preparation services include RNA and DNA extraction from standard and challenging sources as well as sample quality control assessment. The functional genomics applications available are gene expression, array Comparative Genomic Hybridization (aCGH), microRNA, SNP, Chromatin Immunoprecipitation and methylation analysis measured with Affymetrix, lllumina or Agilent microarrays. Microarray validation by Real-Time PCR is also provided. In 2006, the GCL expanded its portfolio of measurement technologies to include Sanger sequencing as well as several Next Generation Sequencing platforms, the PGM from lon Torrent, two HiSeq2000 and a MiSeq from lllumina, a 454FLX and a 454Junior from Roche and two SOLiD5500 from Life Technologies. The availability of these different sequencing technologies enables high-throughput analysis Of a range of genomics applications, such as variant detection, epigenomics, transcriptome sequencing and more. GCL staff also provides basic bioinformatics analysis, training and support. The broad range of services provided by the Genomics Core has supported the research of 129 Investigators in/the past year. During the past grant period the work of the Core has contributed to 484 publications of researchers from 9 research programs. The Genomics Core was chosen as one of the Cancer Genome Characterization Centers and generated Agilent CGH data for 832 glioblastoma multiforme, 1179 ovarian serous cystadenocarcinomas and 244 lung adenocarcinomas. The performance and contribution of the MSKCC TCGA team (clinicians, scientists, computational biologists and genomicists) during the pilot phase helped the Institution obtain a spot in the actual TCGA project that started in 2009, as a Genome Data Analysis center.

基因组学核心实验室（GCL）是一个提供全方位服务的微阵列分析和测序设施。核心 通过提供DNA和RNA分析服务，支持基础、转化和临床研究， 从实验设计和样品制备到基本数据分析和训练。样品制备 服务包括从标准和挑战性来源提取RNA和DNA以及样品质量 控制评估。可用的功能基因组学应用是基因表达、阵列比较、基因组学和基因组学。 基因组杂交（aCGH）、microRNA、SNP、染色质免疫沉淀和甲基化分析 用AffyIron、Illumina或Agilent微阵列测量。实时PCR微阵列验证也是 提供了2006年，GCL扩大了其测量技术组合，包括桑格 测序以及几个下一代测序平台，来自lon Torrent的PGM，两个 来自Illumina的HiSeq2000和MiSeq，来自Roche的454FLX和454Junior以及来自Life的两个SOLiD5500 技术.这些不同测序技术的可用性使得高通量分析成为可能 一系列基因组学应用，如变异检测、表观基因组学、转录组测序和 更多. GCL的工作人员还提供基本的生物信息学分析，培训和支持。的广泛 过去一年，Genomics Core提供的服务支持了129名研究人员的研究。 在过去的赠款期间，核心的工作为来自9个国家的研究人员的484份出版物做出了贡献。 研究项目。基因组学核心被选为癌症基因组表征之一 中心并生成了832例多形性胶质母细胞瘤、1179例卵巢浆液性 囊腺癌和肺腺癌244例。科学、社会和文化理事会的业绩和贡献 TCGA团队（临床医生，科学家，计算生物学家和基因组学家）在试点阶段帮助 该研究所获得了一个在2009年开始的实际TCGA项目中的位置，作为基因组数据分析 中心