Imaging macromolecular machines in gene regulation

我的实验室对了解基因在分子水平上的表达调控很感兴趣。 水平。我们的主要工具是冷冻电子显微镜(Cryo-EM)。我们用低温电子显微镜研究原子 我们感兴趣的大分子机器的运行快照的详细信息。我们已经揭开了 对大分子机器和组件的结构和功能的激动人心的见解 参与(1)蛋白质生产和(2)基因组编辑。我们将继续应用冷冻-EM作为 以及我们剧目中的其他工具，包括特定部位的标记和构象 使用负染色EM进行分析，以产生对结构和功能的机械性见解 涉及基因调控的大分子机器，广义的。在接下来的五年， 我的实验室计划阐明为精确基因奠定基础的分子结构。 蛋白质的表达和生产，细胞的劳动力。结合在 CRISPR基因组工程复合体，这些结果将对 研究人员正在进行转化研究，以对抗无数种疾病。

My laboratory is interested in understanding the regulation of gene expression at the molecular level. Our primary tool is cryo-electron microscopy (cryo-EM). We use cryo-EM to study the atomic details of snapshots of our macromolecular machines of interest in action. We have revealed exciting insights into the structure and function of macromolecular machines and assemblies involved in (1) protein production and (2) genome editing. We will continue to apply cryo-EM as well as other tools in our repertoire, including site-specific labeling and conformational analysis using negative stain EM, to produce mechanistic insights into the structure and function of macromolecular machines involved in gene regulation, broadly-defined. In the next five years, my lab plans on elucidating the molecular architectures that set the foundation for accurate gene expression and production of proteins, the workforce of the cell. Combined with the work on CRISPR genome-engineering complexes, these results will have major implications for researchers performing translational research to combat a myriad of diseases.