Einstein/Rwanda/DRC Consortium for Research in HIV/HPV/ Malignancies

爱因斯坦/卢旺达/刚果民主共和国艾滋病毒/HPV/恶性肿瘤研究联盟

• 批准号: 10654706
• 负责人: Kathryn M. Anastos
• 金额: $ 6.84万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-21 至 2025-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10654706
• 关键词: AIDS related cancer; Address; Africa; Africa South of the Sahara; Antibody titer measurement; Bioinformatics; Biological Assay; Cancer Etiology; Collaborations; Congo; Consent; Data; Democratic Republic of the Congo; Development; Disease; Doctor of Philosophy; Enzyme-Linked Immunosorbent Assay; Epidemic; Fostering; Funding; Funding Opportunities; Genotype; Geography; Goals; HIV; HIV Infections; HPV-High Risk; Histopathology; Human; Human Papilloma Virus-Related Malignant Neoplasm; Human Papillomavirus; Human Resources; Human papilloma virus infection; Human papillomavirus 16; Human papillomavirus 18; Immunoglobulin G; Incidence; Infection; Investigation; Kaposi Sarcoma; Laboratories; Laboratory Research; Lead; Leadership; Malignant Neoplasms; Malignant neoplasm of anus; Malignant neoplasm of cervix uteri; Measurement; Measures; Military Hospitals; Molecular Diagnostic Testing; Non-Hodgkin' s Lymphoma; Pathology; Patients; Persons; Preventative vaccination; Protocols documentation; Research; Research Infrastructure; Research Personnel; Research Project Grants; Resource Sharing; Resource-limited setting; Rwanda; Sampling; Serology; System; Teaching Hospitals; Testing; Time; Translational Research; Universities; University Hospitals; Viral; Viral Load result; Woman; aged; anticancer research; antiretroviral therapy; high risk population; men who have sex with men; microbiome analysis; microbiome research; microbiota; molecular diagnostics; molecular pathology; next generation sequencing; tool; virus related cancer; young woman

PROJECT SUMMARY Sub-Saharan Africa carries a disproportionate burden of HIV, with 69% of infections globally, as well as a high burden of virally induced malignancies, even before the HIV epidemic. Currently a third of all cancers in Sub- Saharan Africa are virally induced. While data from Africa are scant, evidence from other parts of the world indicates that with increasing rates of treatment with combination antiretroviral therapy for persons living with HIV (PLWH), the incidence of HIV-associated malignancies (i.e., Kaposi’s Sarcoma, non-Hodgkin’s Lymphoma) has declined dramatically. However, cancers caused by human papillomavirus (HPV), specifically invasive cervical cancer and anal cancer, have not declined and may have increased. Investigations of HPV-related malignancies are heavily dependent on laboratory testing and histopathology, which can be implemented in resource limited settings. The Rwanda/DRC/Einstein HIV-Associated HPV- Malignancies Research Center (HAMRC) proposed in this application will extend both the geographic and the scientific scope of our research and capacity-building activities. We will leverage our existing HIV research partnerships in the Democratic Republic of Congo (DRC) to include much desired research capacity to assess HPV-related cancers in PLWH. The Center proposes major studies of HPV-related malignancies through two projects: 1) evaluating the impact of HPV prophylactic vaccination in young women living with HIV; and 2) assessing HPV infection and disease in Congolese (DRC) and Rwandan men who have sex with men with and without HIV infection. In both studies we will investigate the interactions of HPV infection and disease with the local microbial flora. The Shared Resources Core addresses the goal of the HAMRC by fostering sustainable research capacity in Rwanda and the DRC to support investigation of viral-associated cancers. Thus, the specific aims of the Shared Resources Core are to provide rigorous molecular diagnostic analyses to support studies of the impact of both HAMRC projects, and to increase capacity to conduct research in HPV-related malignancies in PLWH in Rwanda and the DRC, and will specifically: 1. Replicate in the Pathology Laboratory at Université Protestante au Congo, the real-time PCR platform for hrHPV typing previously established at Rwanda Military Hospital (RMH), in the Einstein/RMH Research Laboratory. 2. Develop at the Rwanda Military Hospital (RMH), in the Einstein/RMH Research Laboratory, and in collaboration with the University of Rwanda, next generation sequencing. 3. Support further development of ELISA testing in Rwanda, at the Einstein/RMH Research Laboratory, using an assay to measure anti-HPV16 and HPV18 IgG antibody titers implementing a protocol established by the Frederick National Laboratory of Cancer Research HPV Serology Laboratory. The proposed capacity building will include personnel development of both research staff and investigators and will efficiently leverage existing research infrastructure previously developed in large part by the efforts of the proposed HARMC leadership.

项目摘要 撒哈拉以南非洲的艾滋病毒的伯恩伯恩比例不成比例，全球感染占69％，而且很高 甚至在艾滋病毒流行之前，实际上引起的恶性肿瘤的负担。目前，所有癌症的三分之一 撒哈拉非洲实际上是诱发的。尽管来自非洲的数据很少，但来自世界其他地区的证据 表明随着患者的抗逆转录病毒疗法的治疗率提高 艾滋病毒（PLWH），与艾滋病毒相关的恶性肿瘤的发生率（即卡波西肉瘤，非霍奇金的肉瘤 淋巴瘤）急剧下降。但是，由人乳头瘤病毒（HPV）引起的癌症，特别是 浸润性宫颈癌和肛门癌尚未下降，可能增加了。 与HPV相关的恶性肿瘤的研究在很大程度上取决于实验室测试和组织病理学， 可以在资源有限的设置中实现。卢旺达/DRC/爱因斯坦HIV相关的HPV- 本应用程序中提出的Malignancys研究中心（HAMRC）将扩展地理和 我们的研究和能力建设活动的科学范围。我们将利用现有的HIV研究 刚果民主共和国（DRC）的合作伙伴关系包括众多期望的研究能力来评估 PLWH中与HPV相关的癌症。 该中心提议通过两个项目对HPV相关的Malignancys进行主要研究：1）评估影响 HPV预防疫苗接种艾滋病毒的年轻妇女； 2）评估HPV感染和疾病 在刚果人（DRC）和与艾滋病毒感染的男人发生性关系的卢旺达男子中。在两项研究中 我们将研究HPV感染和疾病与局部微生物菌群的相互作用。共享 Resources Core通过促进卢旺达的可持续研究能力和 DRC支持与病毒相关的癌症的投资。那，共享的具体目的 资源核心将提供严格的分子诊断分析，以支持对两者的影响的研究 HAMRC项目，并提高在PLWH中进行与HPV相关的恶性肿瘤进行研究的能力 卢旺达和刚果民主共和国，将具体专门： 1。在刚果大学的病理实验室中复制，实时PCR平台 对于先前在爱因斯坦/RMH的卢旺达军事医院（RMH）建立的HRHPV 研究实验室。 2。在爱因斯坦/RMH研究实验室的卢旺达军事医院（RMH）开发 与卢旺达大学合作，下一代测序。 3。支持在爱因斯坦/RMH研究实验室的卢旺达ELISA测试的进一步开发， 使用测定测量抗HPV16和HPV18 IgG抗体滴度实施协议 由弗雷德里克国家癌症研究HPV血清学实验室的国家实验室成立。 拟议的能力建设将包括研究人员和研究人员的人员发展以及 将有效利用以前在很大程度上开发的现有研究基础设施。 拟议的危害领导。