Clinical Ascertainment, Biospecimen Acquisition, Data Management and Analysis Research Core

核心 B 摘要 - 临床确定、保留和生物样本采集研究核心 该核心的目的是发展和支持两个人类出生群体，作为 研究人群以解决有关呼吸道合胞病毒（RSV）对人的影响的基本问题 通过专注于儿童呼吸道疾病和哮喘相互作用途径的科学项目 宿主和病毒对哮喘发展的影响（项目 1），以及可能改变免疫和气道的干预措施 RSV 可能通过上皮细胞发育引起哮喘（项目 2）。本临床测定， 保留和生物样本采集及管理研究核心将用于管理两项人体研究 人群：接触 RSV 后婴儿对肺部感染和哮喘的易感性 (INSPIRE) 和 儿童过敏和新生儿环境 (CANOE)，并管理由此产生的关键生物样本和 数据。数据采集将包括仔细的表型分析、RSV 暴露评估、生理测试、鼻 气道上皮细胞 (NAEC) 采集、鼻刷、血液和外周血单核细胞 (PBMC)，以及项目 1 和 2 的 DNA。 该核心以范德比尔特大学丰富的基础设施和周边儿科中心为中心 和产科诊所进行临床研究。这包括对招聘、注册和 出生队列的保留、纵向随访以及生物样本收集的实验室组成部分 和加工。该核心组建了一个由流行病学家、病毒学家、 具有队列保留经验的免疫学家、遗传学家、生物统计学家和研究人员，以及 生物样本采集并将数据和生物样本提供给分析核心 C，其中数据将被 将进行处理和生物统计分析。该核心分为两个目标，每个目标 支持两个群体之一。首先是促进和监督项目的纵向后续行动。 建立了 INSPIRE 出生队列、生物样本采集，包括项目 1 和 2 的 NAEC。第二个是 促进和监督新 CANOE 出生队列的招募、登记和纵向随访，以及 生物样本采集，包括项目 1 和 2 的 NAEC。

CORE B ABSTRACT - Clinical Ascertainment, Retention and Biospecimen Acquisition Research Core The purpose of this core is to develop and support the two human birth cohorts which serve as the study populations to address fundamental questions about the role of respiratory syncytial virus (RSV) on childhood respiratory morbidity and asthma through scientific projects that focus on interactive pathways of the host and virus on asthma development (Project 1), and interventions that may modify immune and airway epithelial cell development through which RSV may cause asthma (Project 2). This clinical ascertainment, retention and biosample acquisition and management research core will serve to manage the two human study populations: Infant Susceptibility to Pulmonary Infections and Asthma Following RSV Exposure (INSPIRE) and Childhood Allergy and the Neonatal Environment (CANOE), and manage the resultant key biospecimens and data. Data acquisition will include careful phenotyping, RSV exposure assessment, physiologic testing, nasal airway epithelial cell (NAEC) collection, nasal brushings, blood and peripheral blood mononuclear cells (PBMCs), and DNA for Projects 1 and 2. This core is centered around the rich infrastructure at Vanderbilt and surrounding engaged pediatric and obstetrical clinics to conduct the clinical studies. This includes support for the recruitment, enrollment and retention of the birth cohorts, longitudinal follow-up, and the laboratory components of the biosample collection and processing. This core assembles a complementary research team of epidemiologists, virologists, immunologists, geneticists, biostatisticians, and research personnel experienced in cohort retention, and biospecimen acquisition and will provide the data and biospecimens to analytic Core C where data will be processed and biostatistical analyses will be conducted. This core is structured as two aims with each supporting one of the two cohorts. The first is to facilitate and oversee the longitudinal follow-up of the established INSPIRE birth cohort, biospecimen collection, including NAECs for Projects 1 and 2. The second is to facilitate and oversee recruitment, enrollment and longitudinal follow-up of the new CANOE birth cohort, and biospecimen collection, including NAECs for Projects 1 and 2.