Investigating the Cell Division Machinery

总结 细胞分裂是一个复杂的，特别是精心策划的一系列事件， 姐妹染色单体分离成两个细胞。它依赖于大量的蛋白质复合物，蛋白质- 蛋白质相互作用，以及由翻译后活动驱动的调节机制， 修饰酶托雷斯实验室研究的酶和调节机制， 这是以高保真度进行人类细胞分裂所必需的， 比如癌症我们的研究需要一种能够对固定细胞和活细胞进行高分辨率成像的显微镜 空间和时间分辨率。此补充申请资金用于购买Leica云母Widefield 活细胞显微镜，由徕卡显微系统公司制造，以取代目前破碎的 这是托雷斯实验室里的一台显微镜，已经连续使用了12年。请求的 显微镜是必要的，以继续成功地完成父母R35 GM 139539的目标 这是一个有三年以上资金的项目。

SUMMARY Cell division is a complex and specifically orchestrated set of events that culminates in the equal segregation of sister chromatids into two cells. It relies on a multitude of protein complexes, protein- protein interactions, and regulatory mechanisms driven by the activities of posttranslational modification enzymes. The Torres lab investigates the enzymes and regulatory mechanisms that are necessary to carry out human cell division with high fidelity, which are often dysregulated in diseases like cancer. Our research requires a microscope capable of imaging fixed and living cells with high spatial and temporal resolution. This supplement requests funds to purchase a Leica MICA Widefield Live Cell microscope, manufactured by Leica Microsystems, to replace the current broken microscope in the Torres lab that has been continually used for over 12 years. The requested microscope is necessary to continue to successfully complete the aims of the parent R35GM139539 grant, which still has over three years of funding.