Phenotyping and Biospecimen Core

表型鉴定和Biospecimen核心综述 表型鉴定和生物谱系(P&B)核心的主要任务是利用NIA资助的两个 通过筛选百岁老人的研究参与者来确定百岁老人的认知能力 参加RADCO研究的超级老年人、子女和子女配偶，延长他们的 阿尔茨海默病的表型和年度统一神经心理测试、血浆生物标记物 疾病(AD)，以及促进和促进神经成像和死后神经病理学 在有意愿的参与者中进行研究。P&B核心成功实现其六个目标是关键 RADCO对百岁老人及其后代的调查，作为认知韧性和 对认知障碍和阿尔茨海默病的抵抗。这6个具体目标是：具体目标1.确立 认知百岁老人(n=596)，认知正常百岁老人(n=230)， 子女超龄老人(n=480)和子女配偶(n=144)样本带有基线 神经心理评估，其他表型数据，血液样本，遗传和 由NIA资助的两项正在进行的百岁老人研究提供的生物标志物数据。具体目标2.执行 年度新冠肺炎安全虚拟神经认知评估和其他表型数据收集 与劳工组织的研究和LCCP措施保持一致。此外，为了提高数据的准确性， 通过执行跨学科专家诊断共识进行神经认知评估 会议。具体目标3.使用RedCap，管理和执行QC，并使 但RADCO核心和项目收集和生成的所有数据都是安全可用的， 包括神经心理学、神经影像、神经病理表型和生物标志物 转录数据。具体目标4.接受劳工组织+LCCP的研究，然后 纵向采集血样(每年百岁老人、后代和后代配偶 每隔一年)由RADCO参与者提供，供两个项目使用。要将样本保存在 作为可共享资源的生物资源库。具体目标5.支持RADCO神经成像核心 旨在招募RADCO参与者进行局部神经成像并将扫描数据与临床联系起来 数据。具体目标6.招募RADCO参与者进行未来的脑捐赠，确保 死后脑切除和分娩发生得很快，并将临床数据与解剖学联系起来， P&B神经病理学工作产生的病理和分子数据。这些数据将 告知项目1中描绘的弹性和抗性表型。特定脑区组织 将为项目2中的RNA表达研究提供。

Phenotyping and Biospecimen Core Summary The Phenotyping and Biospecimen (P&B) Core’s primary mission is to leverage two NIA-funded centenarian studies by sifting through their study participants in identifying centenarian cognitive superagers, offspring and offspring spouses for enrollment in the RADCO study, extending their phenotyping with annual uniform neuropsychological testing, plasma biomarkers of Alzheimer’s disease (AD), and facilitating and facilitating neuroimaging and post-mortem neuropathological studies among willing participants. The P&B Core's successful conduct of its six aims is key to RADCO’s investigation of centenarians and their offspring as models of cognitive resilience and resistance to cognitive impairment and AD. Those 6 Specific Aims are: Specific Aim 1. Establish a centenarian cognitive superagers (n=596), centenarians with normal cognition (n=230), offspring superagers (n=480) and offspring spouses (n=144) sample with baseline neuropsychological assessments, other phenotypic data, blood samples, and genetic and biomarker data provided by 2 ongoing NIA-funded centenarian studies. Specific Aim 2. Perform annual Covid-19-safe virtual neurocognitive assessments and other phenotypic data collection uniform with the ILO study and LCCP measures. Also, to enhance the accuracy of the neurocognitive assessments by performing interdisciplinary expert diagnoses consensus conferences. Specific Aim 3. Employing REDCap, manage and perform QC, and make easily but securely available all of the data collected and generated by the RADCO Cores and Projects, including the neuropsychological, neuroimaging, neuropathologic phenotype and biomarker and transcriptomic data. Specific Aim 4. To receive from the ILO+LCCP studies and then longitudinally collect blood samples (centenarians annually, offspring, and offspring spouses every other year) from RADCO participants for use by the two projects. To maintain samples in a biorepository as a sharable resource. Specific Aim 5. Support the RADCO Neuroimaging Core’s aims by recruiting RADCO participants for local neuroimaging and linking scan data to clinical data. Specific Aim 6. Recruit RADCO participants for future brain donation, ensure that postmortem brain removal and delivery occur quickly, and link clinical data to anatomic, pathological, and molecular data generated by the P&B neuropathology efforts. These data will inform the resilient and resistant phenotypes delineated in Project 1. Brain area-specific tissues will be provided for RNA expression studies in Project 2.