Phenotyping and Biospecimen Core

表型分析和生物样本核心

• 批准号: 10713948
• 负责人: Stacy Andersen
• 金额: $ 7.8万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-30 至 2026-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10713948
• 关键词: Alzheimer' s Disease; Alzheimer’s disease biomarker; Amyloid beta-42; Anatomy; Apolipoprotein E; Area; Autopsy; Biological; Biological Markers; Blood specimen; Boston; Brain; COVID-19; Centenarian; Clinical Data; Cognition; Cognitive; Consensus; Data; Data Collection; Dedications; Dementia; Diagnosis; Disease Marker; Elderly; Eligibility Determination; Enrollment; Ensure; Excision; Familiarity; Funding; Future; Gene Expression; Genetic; Genetic Transcription; Glial Fibrillary Acidic Protein; Impaired cognition; Impairment; Investigation; Knowledge Portal; Lead; Light; Link; Longevity; Los Angeles; Magnetic Resonance Imaging; Measures; Mission; Modeling; Molecular; Nerve Degeneration; Neurocognitive; Neuropsychological Tests; Neuropsychology; New York City; Outcome; Participant; Pathologic; Patient Recruitments; Phenotype; Plasma; RNA; Research Personnel; Resistance; Resources; Sampling; Scanning; Secure; Spouses; Statistical Data Interpretation; System; Tissues; Universities; Work; biobank; cognitive function; cytokine; data management; data sharing; exome; experience; innovation; neurofilament; neuroimaging; neuropathology; offspring; participant enrollment; phenotypic data; protective factors; recruit; resilience; success; symposium; tool; transcriptomics; translational potential; virtual

Phenotyping and Biospecimen Core Summary The Phenotyping and Biospecimen (P&B) Core’s primary mission is to leverage two NIA-funded centenarian studies by sifting through their study participants in identifying centenarian cognitive superagers, offspring and offspring spouses for enrollment in the RADCO study, extending their phenotyping with annual uniform neuropsychological testing, plasma biomarkers of Alzheimer’s disease (AD), and facilitating and facilitating neuroimaging and post-mortem neuropathological studies among willing participants. The P&B Core's successful conduct of its six aims is key to RADCO’s investigation of centenarians and their offspring as models of cognitive resilience and resistance to cognitive impairment and AD. Those 6 Specific Aims are: Specific Aim 1. Establish a centenarian cognitive superagers (n=596), centenarians with normal cognition (n=230), offspring superagers (n=480) and offspring spouses (n=144) sample with baseline neuropsychological assessments, other phenotypic data, blood samples, and genetic and biomarker data provided by 2 ongoing NIA-funded centenarian studies. Specific Aim 2. Perform annual Covid-19-safe virtual neurocognitive assessments and other phenotypic data collection uniform with the ILO study and LCCP measures. Also, to enhance the accuracy of the neurocognitive assessments by performing interdisciplinary expert diagnoses consensus conferences. Specific Aim 3. Employing REDCap, manage and perform QC, and make easily but securely available all of the data collected and generated by the RADCO Cores and Projects, including the neuropsychological, neuroimaging, neuropathologic phenotype and biomarker and transcriptomic data. Specific Aim 4. To receive from the ILO+LCCP studies and then longitudinally collect blood samples (centenarians annually, offspring, and offspring spouses every other year) from RADCO participants for use by the two projects. To maintain samples in a biorepository as a sharable resource. Specific Aim 5. Support the RADCO Neuroimaging Core’s aims by recruiting RADCO participants for local neuroimaging and linking scan data to clinical data. Specific Aim 6. Recruit RADCO participants for future brain donation, ensure that postmortem brain removal and delivery occur quickly, and link clinical data to anatomic, pathological, and molecular data generated by the P&B neuropathology efforts. These data will inform the resilient and resistant phenotypes delineated in Project 1. Brain area-specific tissues will be provided for RNA expression studies in Project 2.

表型分析和生物样本核心摘要 表型分析和生物样本 (P&B) 核心的主要任务是利用 NIA 资助的两项 百岁老人研究通过筛选研究参与者来识别百岁老人的认知 超级老人、后代和后代配偶参加 RADCO 研究，延长他们的 每年统一的神经心理学测试、阿尔茨海默病的血浆生物标志物进行表型分析 疾病（AD），并促进和促进神经影像学和死后神经病理学 自愿参与者之间的研究。 P&B 核心成功实现其六大目标是实现这一目标的关键 RADCO 对百岁老人及其后代作为认知弹性模型的调查 抵抗认知障碍和AD。这 6 个具体目标是： 具体目标 1. 建立 认知超级老人（n=596）、认知正常的百岁老人（n=230）、 具有基线的后代超级老人 (n=480) 和后代配偶 (n=144) 样本 神经心理学评估、其他表型数据、血液样本以及遗传和 生物标志物数据由两项正在进行的 NIA 资助的百岁老人研究提供。具体目标 2. 执行 年度 Covid-19 安全虚拟神经认知评估和其他表型数据收集 与国际劳工组织研究和 LCCP 措施保持一致。另外，为了提高准确率 通过执行跨学科专家诊断共识进行神经认知评估 会议。具体目标 3. 采用 REDCap，管理和执行 QC，轻松制作 但可以安全地使用 RADCO 核心和项目收集和生成的所有数据， 包括神经心理学、神经影像学、神经病理表型和生物标志物 转录组数据。具体目标 4. 接收 ILO+LCCP 研究成果，然后 纵向采集血样（每年百岁老人、后代、后代配偶 每隔一年）由 RADCO 参与者提供，供两个项目使用。将样品保存在 生物样本库作为一种可共享的资源。具体目标 5. 支持 RADCO 神经影像核心 旨在招募 RADCO 参与者进行局部神经影像检查并将扫描数据与临床联系起来 数据。具体目标 6. 招募 RADCO 参与者进行未来的大脑捐赠，确保 死后大脑切除和交付发生得很快，并将临床数据与解剖学数据联系起来， P&B 神经病理学工作生成的病理和分子数据。这些数据将 告知项目 1 中描述的弹性和抵抗表型。大脑区域特定组织 将用于项目 2 中的 RNA 表达研究。