Structure, Mechanism, and Regulation of PACAP/VIP GPCR Subtypes

PACAP/VIP GPCR 亚型的结构、机制和调控

• 批准号: 10819926
• 负责人: Jianing Li
• 金额: $ 35.32万
• 依托单位: PURDUE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10819926
• 关键词: Structure; Mechanism; Regulation; PACAP; VIP

Among the pharmacologically important Class B G protein-coupled receptors (GPCRs), the PAC1/VPAC receptors (ADCYAP1R1/VIPR1/VIPR2) for pituitary adenylate cyclase activated polypeptide (PACAP, ADCYAP1) and vasoactive intestinal peptide (VIP) have been implicated in several disorders, including chronic pain and stress-related behavioral abnormalities which are among the most prevalent global neurological challenges today. However, the development of reagents targeting these receptors for potential therapeutics has been hampered by the lack of full-length PAC1/VPAC receptor structural information, and mechanistic understandings of how ligand binding can differentially drive conformational changes for receptor activation and biased signaling. Accordingly, the overarching aim of this proposal is to employ state-of-the-art computational methods to model PAC1/VPAC receptor structure and dynamics, with the goal of developing small-molecule compounds to modulate their functions. For each receptor subtype, the identification of unique structural features that allow for specific ligand interactions and transducer protein associations will facilitate the rational design and optimization of small-molecule ligands. Towards that goal, three specific aims will be pursued: (1) to model and compare structures and mechanisms that determine neuropeptide selectivity and function among the physiologically relevant PAC1Null, PAC1Hop1, VPAC1, and VPAC2 receptor subtypes; (2) to delineate PAC1/VPAC receptor-transducer protein interactions and specificity; and (3) to develop and optimize small molecules for selective PAC1 and VPAC receptor regulation. Our preliminary data have established the modeling methodology with the PAC1Null receptor, demonstrated distinct signaling behaviors of the PAC1/VPAC receptors, and identified two different PAC1Null antagonists. Under Aims 1 and 2, we will use homology modeling, protein structure refinement, and molecular dynamics simulations to study the receptors that are ligand-free and complexed with a neuropeptide (PACAP/VIP) or a transducer protein (Gs/Gq/β-arrestins). In particular, we will elucidate the binding sites that are key for ligand specificity as well as conformational states that facilitate long-term signaling. Receptor mutagenesis and constructs will inform and/or substantiate the receptor models. Under Aim 3, we will integrate molecular docking and simulations, organic synthesis, and molecular and cellular assays to develop selective small-molecule modulators. Especially, the strategies to target the orthosteric and allosteric sites will be tested. Our multidisciplinary approach is innovative as it provides an unparalleled and comprehensive means to investigate the PAC1/VPAC receptors in various conditions and functional states. Further, the proposed research is significant, because it will close fundamental gaps in understanding how molecular structures and dynamics can dictate PAC1/VPAC receptor mechanisms. Finally, given current limitations in therapeutics, these studies may offer new opportunities and approaches to treat challenging neurological disorders.

在非常重要的B类G蛋白偶联受体（GPCR）中，PAC 1/VPAC 垂体腺苷酸环化酶激活多肽（PACAP， ADCYAP 1）和血管活性肠肽（VIP）与几种疾病有关，包括慢性炎症和慢性炎症。 疼痛和压力相关的行为异常，这是最普遍的全球神经系统疾病之一， 今天的挑战。然而，针对这些受体的潜在治疗剂的开发 由于缺乏全长PAC 1/VPAC受体结构信息， 了解配体结合如何不同地驱动受体激活的构象变化 和偏置信号。因此，本提案的总体目标是采用最先进的 计算方法来模拟PAC 1/VPAC受体的结构和动力学，目标是开发 小分子化合物来调节它们的功能。对于每种受体亚型， 允许特异性配体相互作用和转导蛋白缔合的结构特征将促进 小分子配体的合理设计和优化。为实现这一目标，将有三个具体目标： 追求：（1）建模和比较决定神经肽选择性的结构和机制， 生理学相关的PAC 1 β、PAC 1Hop 1、VPAC 1和VPAC 2受体亚型之间的功能;（2） 描述PAC 1/VPAC受体-转导蛋白的相互作用和特异性;（3）开发和 优化选择性PAC 1和VPAC受体调节的小分子。我们的初步数据显示 建立了PAC 1 β受体的建模方法，展示了不同的信号传导行为 的PAC 1/VPAC受体，并确定了两种不同的PAC 1受体拮抗剂。根据目标1和2，我们将 使用同源建模，蛋白质结构细化和分子动力学模拟来研究 无配体并与神经肽（PACAP/VIP）或转导蛋白复合的受体 (Gs/Gq/β-抑制蛋白）。特别是，我们将阐明的结合位点是关键的配体特异性，以及 促进长期信号传导的构象状态。受体诱变和构建体将告知 和/或证实受体模型。在目标3下，我们将整合分子对接和模拟， 有机合成，以及分子和细胞分析，以开发选择性小分子调节剂。 特别是，将测试靶向正构和变构位点的策略。我们的多学科 方法是创新的，因为它提供了一个无与伦比的和全面的手段来调查 PAC 1/VPAC受体在各种条件和功能状态。此外，拟议的研究是 意义重大，因为它将填补理解分子结构和动力学如何 可以决定PAC 1/VPAC受体机制。最后，鉴于目前治疗方法的局限性，这些研究 可能为治疗具有挑战性的神经系统疾病提供新的机会和方法。

项目成果

Molecular Basis of Class B GPCR Selectivity for the Neuropeptides PACAP and VIP.

• DOI: 10.3389/fmolb.2021.644644
• 发表时间: 2021
• 期刊: Frontiers in molecular biosciences
• 影响因子: 5
• 作者: Liao C;Remington JM;May V;Li J
• 通讯作者: Li J

PAC1 Receptors: Shapeshifters in Motion.

• DOI: 10.1007/s12031-018-1132-0
• 发表时间: 2019-07
• 期刊: Journal of molecular neuroscience : MN
• 作者: Liao C;May V;Li J
• 通讯作者: Li J

Aggregation State of Synergistic Antimicrobial Peptides.

• DOI: 10.1021/acs.jpclett.0c02094
• 发表时间: 2020-11-05
• 期刊: The journal of physical chemistry letters
• 作者: Remington JM;Liao C;Sharafi M;Ste Marie EJ;Ferrell JB;Hondal RJ;Wargo MJ;Schneebeli ST;Li J
• 通讯作者: Li J

Selective Monofunctionalization Enabled by Reaction-History-Dependent Communication in Catalytic Rotaxanes.

• DOI: 10.1002/anie.202006305
• 发表时间: 2020-09-14
• 期刊: Angewandte Chemie (International ed. in English)
• 作者: Rajappan SC;McCarthy DR;Campbell JP;Ferrell JB;Sharafi M;Ambrozaite O;Li J;Schneebeli ST
• 通讯作者: Schneebeli ST

Assessment of Conformational State Transitions of Class B GPCRs Using Molecular Dynamics.

• DOI: 10.1007/978-1-4939-9121-1_1
• 发表时间: 2019
• 期刊: Methods in molecular biology
• 作者: Chenyi Liao;V. May;Jianing Li