Core 3: Biocontainment Research Support Services Core

核心 3：生物防护研究支持服务核心

• 批准号: 10791950
• 负责人: Colin S. Duckett
• 金额: $ 102.95万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-08-18 至 2028-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10791950
• 关键词: 2019-nCoV; Animal Experimentation; Animal Model; Animals; Antibodies; Antigens; Applied Research; Area; Award; Basic Science; Biological Assay; Biological Markers; Cell Separation; Cells; Characteristics; Chikungunya virus; Clinical; Collaborations; Communities; Containment; Cytomegalovirus; Development; Diagnostic; Disease; Ebola; Ensure; Environment; Equipment and supply inventories; Evaluation; Fee-for-Service Plans; Ferrets; Flow Cytometry; Francisella tularensis; Funding; Goals; HIV; Hamsters; Home; Human; Immune; Immunologic Markers; Immunologic Monitoring; Immunology; Immunology procedure; Infection; Influenza; Infrastructure; Institution; Laboratories; Laboratory Animal Models; Malaria; Microbiology; Mission; Modeling; Mus; Mycobacterium tuberculosis; National Institute of Allergy and Infectious Disease; Performance; Pharmaceutical Preparations; Productivity; Publications; Recording of previous events; Research; Research Personnel; Research Support; Resource Development; Safety; Sampling; Secure; Services; Societies; Sorting; Source; Therapeutic; Training; Training Support; Translational Research; Tuberculosis; United States National Institutes of Health; Universities; Vaccines; Variant; Viral; Virus; Workforce Development; Yellow fever virus; Yersinia pestis; ZIKA; biocontainment facility; biodefense; cytokine; disease diagnostic; experience; global health; laboratory experience; mouse model; novel; novel therapeutics; novel vaccines; novel virus; operation; pathogen; pathogenic virus; programs; virology

ABSTRACT- Biocontainment Research Support Services Core (Core 3) The central mission of the Duke RBL is to support the basic and translational research necessary to develop drugs, diagnostics, and vaccines for emerging/reemerging infections and biodefense. Support through this award will allow us to expand our existing services to refine our small animal models programs, thus providing a wider range of services offered to the research community. It will also render our virology program more effective, particularly through the development of a direct workflow from our clinical laboratories at Duke, in addition to relying on third party sources, particularly for rapidly emerging viral variants. Thirdly, we will broaden our range of immunological assays performed in our BSL-3 suites, such as live cell flow sorting and rapid evaluation of cytokine and biomarker profiles. The goals of this Biocontainment Research Support Services Core (Core 3) are to 1) establish, maintain, and provide small animal challenge models with BSL-3 pathogens through the Duke RBL Animal Models Research Support Services; 2) maintain and provide quality- controlled virus inventory of Select Agents and pathogens requiring BSL-3 conditions through the Duke RBL Virology Research Support Services; and 3) provide comprehensive immune monitoring at BSL-3 via humoral, cellular, and biomarker analysis through the Duke RBL Immunology and Flow Cytometry Research Support Services. Implementation of these three Aims will allow us to expand the capabilities and services that we can offer not only to the Duke community, but also to our neighboring institutions and the national RBL network.

摘要-生物防护研究支持服务核心（核心3） 杜克RBL的中心使命是支持发展所需的基础和转化研究。 用于新发/再发感染和生物防御的药物、诊断和疫苗。支持通过此 该奖项将使我们能够扩大我们现有的服务，以完善我们的小动物模型计划，从而提供 为研究界提供更广泛的服务。这也将使我们的病毒学项目 有效，特别是通过我们在杜克的临床实验室开发的直接工作流程， 除了依赖第三方来源之外，特别是对于快速出现的病毒变体。第三，我们将 扩大了我们在BSL-3套件中进行的免疫学检测范围，例如活细胞流式分选， 快速评估细胞因子和生物标志物谱。生物防护研究支持的目标是 服务核心（核心3）是1）建立、维护和提供BSL-3小动物攻毒模型 病原体通过杜克RBL动物模型研究支持服务; 2）保持和提供质量- 通过杜克RBL控制需要BSL-3条件的特定病原体和病原体的病毒库存 病毒学研究支持服务;和3）通过体液， 通过杜克RBL免疫学和流式细胞术研究支持进行细胞和生物标志物分析 服务这三个目标的实现将使我们能够扩大我们所能提供的能力和服务， 不仅提供给杜克社区，也提供给我们的邻近机构和国家RBL网络。