BSL3 management and practice of infectious disease research at HTRL

项目摘要(总体) 我们国家检测、预防和打击生物恐怖主义和新出现的传染病的能力取决于 通过对致病微生物的生物医学研究和理解而产生的技术 人类对感染的免疫反应。在2001年发生生物恐怖主义事件后，国家过敏研究所 和传染病建立了区域生物控制实验室(RBL)网络，以促进安全 在12个生物安全3级(BSL3)实验室进行研究。反过来，托管这些RBL的机构和大学 持续管理这些设施，培养强大的生物安全文化，并推动研究询问 微生物引起人类疾病的分子机制。芝加哥大学主办了 区域生物遏制霍华德·泰勒·里基茨实验室(HTRL)由其部门运营 自2009年以来一直从事微生物学研究。当这个国家开始实施关闭以防止COVID-19传播时， 高铁的基本专业人员继续进行关键管理业务，并将他们的专业知识用于研究 SARS-CoV2。如果没有现有的专业知识和奉献精神，这种势头是不可能的 工程师、生物安全官员、兽医人员、科学家以及来自大学的财政支持。这个 这项提议的目标是提出一项计划，以维持BSL3空间的安全运行和管理 区域生物遏制HTRL，并继续培训将提供研究支持的专业人员 为制定必要的措施以减轻和保护公众健康免受传染病感染提供的服务 疾病可能是故意的、意外的或自然发生的健康紧急情况的结果。这个计划 将由三个核心实现。核心1设施管理、维护和运营 (C1Facilities@HTRL)，由George Langan领导，描述了支持持续遏制BSL3的活动 通过对建筑物进行净化、重新测试、认证、预防性维护、维修或更换 系统和遵守生物安全、环境健康和生物安全法规。核心2 BSL3 由Joseph Kanabrocki领导的实践(C2BSL3Practice@HTRL)描述了培训 人员和标准操作程序的制定，以及维持安全和 安全的环境。核心3生物遏制研究支持服务(C3ResearchSupport@HTRL) 由Dominique Missiakas领导，描述了研究风险组3和选择代理的研究能力 病原体。HTRL将使用一个基于网络的门户网站来做广告和提供研究服务，如隔离和 利用体外和体内试验鉴定病原体、毒力和对策研究 动物模型。RBL董事之间的每月虚拟会议和年度面对面会议将确定 协作、改进和交叉培训的机会。这些交互作用将扩展容量 将RBL网络作为一个整体，以应对公共卫生突发事件、评估威胁和 应对措施，并提供符合生物安全标准的教育和培训。

PROJECT ABSTRACT (OVERALL) Our nation’s ability to detect, prevent and counter bioterrorism and emerging infectious diseases depends on technologies that are generated through biomedical research on disease-causing microbes and understanding human immune responses to infection. Following bioterrorism events in 2001, the National Institute of Allergy and Infectious Diseases established the Regional Biocontainment Laboratory (RBL) Network, to promote safe research in 12 Biosafety Level 3 (BSL3) laboratories. In turn, Institutions and Universities hosting these RBLs sustained the management of these facilities, fostered a strong Biosafety culture, and fueled research inquiries into the molecular mechanisms whereby microbes cause human disease. The University of Chicago hosts the Regional Biocontainment Howard Taylor Ricketts Laboratory (HTRL) operated by its Department of Microbiology since 2009. When this nation began to implement shutdowns to prevent the spread of COVID-19, essential professionals at the HTRL continued key management operations and pivoted their expertise to study SARS-CoV2. This momentum would not have been possible without the existing expertise and dedication of engineers, biosafety officers, veterinary staff, scientists, as well as financial support from the University. The goal of this proposal is to present a plan to maintain the safe operation and management of the BSL3 space of the Regional Biocontainment HTRL and to continue training professionals who will provide research support services for the development of measures necessary to mitigate and protect the public's health from infectious diseases that may be the result of intentional, accidental, or naturally occurring health emergency. This plan will be implemented by three cores. Core 1 Facility Management, Maintenance and Operations (C1Facilities@HTRL) led by George Langan, describes activities that support continuous BSL3 containment through decontamination, retesting, certification, preventive maintenance, repair or replacement of building systems and compliance with Biosecurity, Environmental Health, and Biosafety regulations. Core 2 BSL3 Practices (C2BSL3Practice@HTRL) led by Joseph Kanabrocki, describes activities for the training of personnel and the development of Standard Operating Procedures as well as activities to sustain a safe and secure environment. Core 3 Biocontainment Research Support Services (C3ResearchSupport@HTRL) led by Dominique Missiakas, describes research capabilities for the study of Risk Group 3 and Select Agent pathogens. HTRL will use a web-based portal to advertise and offer research services such as isolation and characterization of pathogens, virulence and countermeasure studies using in vitro and in vivo assays and animal models. Monthly virtual and annual in-person meetings between RBL Directors will identify opportunities for collaborations, improvements, and cross-training. These interactions will expand the capacity of the RBL network as a whole to respond to public health emergencies, conduct evaluations of threats and countermeasures, and provide education and training to conform with biosafety standards.