BSL3 management and practice of infectious disease research at HTRL

HTRL 传染病研究的 BSL3 管理和实践

• 批准号: 10793948
• 负责人: JOSEPH A. KANABROCKI
• 金额: $ 246.91万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-08-18 至 2028-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10793948
• 关键词: 2019-nCoV; Accidents; Air; Animal Model; Antibiotic Therapy; Antibiotics; Articulation; Awareness; Bacteria; Bacterial Infections; Biological; Biological Assay; Biomedical Research; Bioterrorism; Birds; COVID-19; COVID-19 pandemic; Certification; Chicago; Classification; Clostridium difficile; Collaborations; Communicable Diseases; Communities; Containment; Coronavirus; Country; Decontamination; Dedications; Dengue; Development; Disease; Doctor of Philosophy; Ebola virus; Economics; Emergency Situation; Emerging Communicable Diseases; Engineering; Environment; Environmental Health; Epidemic; Equilibrium; Evaluation; Event; Financial Support; Fostering; Frequencies; Funding Opportunities; Genome; Goals; Health; Human; Human Resources; Immune response; Immunity; In Vitro; Infection; Infectious Agent; Infectious Diseases Research; Influenza; Institution; Investigation; Knowledge; Laboratories; Laboratory Personnel; Leadership; Livestock; Maintenance; Mammals; Measures; Medical; Microbe; Microbiology; Middle East Respiratory Syndrome Coronavirus; Modeling; Molecular; Morbidity - disease rate; Mutation; National Institute of Allergy and Infectious Disease; Operations Research; Panic; Pathogenicity; Persons; Pharmaceutical Preparations; Population; Preventive; Procedures; Protective Devices; Protocols documentation; Public Health; RNA Viruses; Regulation; Research; Research Activity; Research Support; Resources; Risk; Role; SARS coronavirus; Safety; Scientist; Secure; Services; Societies; Structure; System; Technology; Testing; Therapeutic Intervention; Toxin; Training; Training Activity; Training and Education; Training and Infrastructure; Universities; Vaccines; Variant; Viral; Virulence; Virus; ZIKA; Zoonoses; biodefense; biosafety level 3 facility; biosecurity; chikungunya; combat; companion animal; cost; drug resistant pathogen; hazard; human disease; improved; in vivo; insight; medical countermeasure; meetings; methicillin resistant Staphylococcus aureus; microbial; operation; pathogen; pathogenic microbe; prevent; preventive intervention; professor; public health emergency; repaired; response; transmission process; viral outbreak; virtual; web portal

PROJECT ABSTRACT (OVERALL) Our nation’s ability to detect, prevent and counter bioterrorism and emerging infectious diseases depends on technologies that are generated through biomedical research on disease-causing microbes and understanding human immune responses to infection. Following bioterrorism events in 2001, the National Institute of Allergy and Infectious Diseases established the Regional Biocontainment Laboratory (RBL) Network, to promote safe research in 12 Biosafety Level 3 (BSL3) laboratories. In turn, Institutions and Universities hosting these RBLs sustained the management of these facilities, fostered a strong Biosafety culture, and fueled research inquiries into the molecular mechanisms whereby microbes cause human disease. The University of Chicago hosts the Regional Biocontainment Howard Taylor Ricketts Laboratory (HTRL) operated by its Department of Microbiology since 2009. When this nation began to implement shutdowns to prevent the spread of COVID-19, essential professionals at the HTRL continued key management operations and pivoted their expertise to study SARS-CoV2. This momentum would not have been possible without the existing expertise and dedication of engineers, biosafety officers, veterinary staff, scientists, as well as financial support from the University. The goal of this proposal is to present a plan to maintain the safe operation and management of the BSL3 space of the Regional Biocontainment HTRL and to continue training professionals who will provide research support services for the development of measures necessary to mitigate and protect the public's health from infectious diseases that may be the result of intentional, accidental, or naturally occurring health emergency. This plan will be implemented by three cores. Core 1 Facility Management, Maintenance and Operations (C1Facilities@HTRL) led by George Langan, describes activities that support continuous BSL3 containment through decontamination, retesting, certification, preventive maintenance, repair or replacement of building systems and compliance with Biosecurity, Environmental Health, and Biosafety regulations. Core 2 BSL3 Practices (C2BSL3Practice@HTRL) led by Joseph Kanabrocki, describes activities for the training of personnel and the development of Standard Operating Procedures as well as activities to sustain a safe and secure environment. Core 3 Biocontainment Research Support Services (C3ResearchSupport@HTRL) led by Dominique Missiakas, describes research capabilities for the study of Risk Group 3 and Select Agent pathogens. HTRL will use a web-based portal to advertise and offer research services such as isolation and characterization of pathogens, virulence and countermeasure studies using in vitro and in vivo assays and animal models. Monthly virtual and annual in-person meetings between RBL Directors will identify opportunities for collaborations, improvements, and cross-training. These interactions will expand the capacity of the RBL network as a whole to respond to public health emergencies, conduct evaluations of threats and countermeasures, and provide education and training to conform with biosafety standards.

项目摘要（总体） 我们国家检测，预防和反对生物恐怖主义和新兴传染病的能力取决于 通过生物医学研究产生的有关疾病的微生物和理解的技术 人类免疫复杂引起感染。在2001年的生物恐怖事件之后，美国国家过敏研究所 传染病建立了区域生物疗养实验室（RBL）网络，以促进安全 研究12级生物安全3（BSL3）实验室的研究。反过来，托管这些RBL的机构和大学 维持这些设施的管理，培养了强大的生物安全文化，并加剧了研究调查 进入微生物引起人类疾病的分子机制。芝加哥大学主持 区域生物疗法霍华德·泰勒·里基茨实验室（HTRL）由其部门运营 自2009年以来的微生物学。当这个国家开始实施关闭以防止Covid-19的传播时， HTRL的基本专业人士继续进行关键管理运营，并枢纽以研究 SARS-CoV-2。没有现有的专业知识和奉献精神，这种势头是不可能的 工程师，生物安全人员，兽医，科学家以及大学的财务支持。 该建议的目标是提出一项计划，以维护BSL3空间的安全操作和管理 区域生物内在HTRL，并继续培训将提供研究支持的专业人员 为制定减轻和保护公众健康免受感染性所必需的措施的服务 可能是故意，意外或自然发生紧急情况的疾病。这个计划 将由三个核心实施。核心1设施管理，维护和操作 （c1facities@htrl）由乔治·兰加（George Langan）领导，描述了支持连续BSL3遏制的活动 通过去污，重新测试，认证，预防性维护，修理或更换建筑物 系统和遵守生物学，环境健康和生物安全法规。核心2 BSL3 约瑟夫·卡纳布罗基（Joseph Kanabrocki）领导的实践（C2BSL3PRACTICE@htrl）描述了培训的活动 人员和标准操作程序的制定以及维持安全和安全的活动 安全环境。核心3生物疗法研究支持服务（C3ResearchSupport@htrl） 由多米尼克·米西亚卡斯（Dominique Missiakas）领导，描述了风险研究3和精选代理的研究能力 病原体。 HTRL将使用基于Web的门户来宣传和提供研究服务，例如隔离和 使用体外和体内测定法和病原体，毒力和对策研究的表征 动物模型。 RBL董事之间的每月虚拟和年度面对面会议将确定 合作，改进和交叉培训的机会。这些互动将扩大能力 RBL网络的整体响应公共卫生紧急情况，对威胁进行评估和 对策，并提供教育和培训，以符合生物安全标准。