Elucidating the mechanism behind oscillation between glycolysis and gluconeogenesis

阐明糖酵解和糖异生之间振荡背后的机制

• 批准号: 10807519
• 负责人: Junyoung O. Park
• 金额: $ 1.14万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-07-01 至 2026-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10807519
• 关键词: Award; Biochemical; Biochemical Reaction; Biological Assay; Cell Culture Techniques; Environment; Funding; Future; Genomics; Gluconeogenesis; Glucose; Glycolysis; Goals; Human; Investigation; Mammalian Cell; Mass Spectrum Analysis; Measurement; Measures; Mentors; Metabolic; Metabolism; Nutrient; Outcomes Research; Parents; Regulation; Research; Research Personnel; Science; Talents; Training; blood glucose regulation; doctoral student; experimental study; metabolic phenotype; parent grant; skills; summer program; symposium; undergraduate student

Project Summary Metabolism is a dynamic network of biochemical reactions capable of adapting to changing environments. In changing glucose availability, glycolysis and gluconeogenesis support systemic glucose homeostasis. In the parent award, we investigate the regulation and coordination of glycolysis and gluconeogenesis in mammalian cells under dynamic nutrient environments. The goal of this supplement application is to acquire funding to support one talented and motivated undergraduate student researcher for 10 weeks in the summer of 2023. She will measure the rates of glycolysis and gluconeogenesis in mammalian cells under dynamic nutrient environments by employing biochemical assays and mass spectrometry. She will be mentored by the PI and one PhD student who will train her to perform cell culture experiments, metabolic measurements, and analyze results. She will join the Bruins-In-Genomics (BIG) Summer Program on UCLA campus to broaden her scientific horizons and develop professional skills. She will share her research outcomes with the PI’s research group and other researchers on campus by presenting her project at the 2023 BIG Research Symposium. These activities will further the undergraduate student researcher’s passion for biomedical science and act as a steppingstone for her to become a future STEM leader.

项目总结

项目成果

Toward Systems-Level Metabolic Analysis in Endocrine Disorders and Cancer.

• DOI: 10.3803/enm.2023.1814
• 发表时间: 2023-12
• 期刊: Endocrinology and metabolism (Seoul, Korea)
• 作者: Lakhani A;Kang DH;Kang YE;Park JO
• 通讯作者: Park JO

Determination of Metabolic Fluxes by Deep Learning of Isotope Labeling Patterns.

通过同位素标记模式的深度学习确定代谢通量。

• DOI: 10.1101/2023.11.06.565907
• 发表时间: 2023
• 期刊: bioRxiv : the preprint server for biology
• 作者: Law,RichardC;O'Keeffe,Samantha;Nurwono,Glenn;Ki,Rachel;Lakhani,Aliya;Lai,Pin-Kuang;Park,JunyoungO
• 通讯作者: Park,JunyoungO