Elucidating the mechanism behind oscillation between glycolysis and gluconeogenesis

项目摘要 新陈代谢是一个动态的生化反应网络，能够适应变化的环境。在……里面 改变葡萄糖的可获得性、糖酵解和糖异生有助于维持全身的葡萄糖稳态。在 父母奖，我们研究了哺乳动物糖酵解和糖异生的调节和协调 细胞在动态的营养环境下。本补充申请的目标是获得资金，以 在2023年夏天，支持一位才华横溢、积极进取的本科生研究员10周。她 将测量在动态营养条件下哺乳动物细胞糖酵解和糖异生的速率 使用生化分析和质谱学的方法。她将由私家侦探和 一名博士生将培训她进行细胞培养实验、新陈代谢测量和分析 结果。她将加入加州大学洛杉矶分校的布鲁因基因组学(BIG)夏季计划，以扩大她的科学研究范围 扩大视野，培养专业技能。她将与PI的研究小组分享她的研究成果，并 通过在2023年大型研究研讨会上展示她的项目，校园里的其他研究人员也这样做了。这些活动 将进一步促进本科生研究人员对生物医学科学的热情，并充当跳板 让她成为未来的STEM领袖。

Project Summary Metabolism is a dynamic network of biochemical reactions capable of adapting to changing environments. In changing glucose availability, glycolysis and gluconeogenesis support systemic glucose homeostasis. In the parent award, we investigate the regulation and coordination of glycolysis and gluconeogenesis in mammalian cells under dynamic nutrient environments. The goal of this supplement application is to acquire funding to support one talented and motivated undergraduate student researcher for 10 weeks in the summer of 2023. She will measure the rates of glycolysis and gluconeogenesis in mammalian cells under dynamic nutrient environments by employing biochemical assays and mass spectrometry. She will be mentored by the PI and one PhD student who will train her to perform cell culture experiments, metabolic measurements, and analyze results. She will join the Bruins-In-Genomics (BIG) Summer Program on UCLA campus to broaden her scientific horizons and develop professional skills. She will share her research outcomes with the PI’s research group and other researchers on campus by presenting her project at the 2023 BIG Research Symposium. These activities will further the undergraduate student researcher’s passion for biomedical science and act as a steppingstone for her to become a future STEM leader.