Safeguarding Genetic Resources of Aquatic Biomedical Models

保护水生生物医学模型遗传资源

• 批准号: 10808269
• 负责人: Maria Teresa Gutierrez-Wing
• 金额: $ 23.39万
• 依托单位: LOUISIANA STATE UNIV AGRICULTURAL CENTER
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-06-15 至 2025-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10808269
• 关键词: 10 year old; Address; Algae; Ambystoma; Animals; Aplysia; Aplysia species; Award; Basic Science; Biocompatible Materials; Biological; Biological Models; Communities; Community Developments; Consensus; Cryopreservation; Database Management Systems; Development; Documentation; Educational Curriculum; Educational workshop; Embryo; Engineering; Ensure; Equipment; Event; Evolution; Fertilization; Freezing; Funding; Future; Genetic; Genetic Models; Genetic Variation; Germ; Growth; Human Resources; Hurricane; Individual; Industrialization; Information Systems; Internet; Kentucky; Knowledge; Label; Laboratories; Larva; Life; Link; Liquid substance; Location; Maintenance; Methods; Microfluidics; Modeling; Nitrogen; Oocytes; Parents; Pathway interactions; Positioning Attribute; Process; Productivity; Protocols documentation; Rana; Recommendation; Recovery; Reproducibility; Research; Research Infrastructure; Resources; Salamander; Sampling; Schedule; Secure; Services; Site; Somatic Cell; Standardization; System; Technology; Training; Training Programs; United States National Institutes of Health; Universities; Vendor; Work; Xenopus; Xiphophorus; Zebrafish; aquatic organism; base; cost; egg; genetic information; genetic resource; improved; innovation; instrument; marine; member; model organism; next generation; novel strategies; operation; outreach; outreach program; parent grant; preservation; pressure; programs; protocol development; repository; response; seal; sperm cell; stem cells; success

Project Summary/Abstract: The activities at the Aquatic Germplasm and Genetic Resources Center (AGGRC) have expanded considerably over the past 5 years with us now addressing repository development for all five NIH-funded aquatic stock centers. Reliable availability of liquid nitrogen is essential to most of that work. Baton Rouge has a strong industrial base, and liquid nitrogen is available from local suppliers. However, delivery and on-site management have become increasingly problematic and are a growing constraint to our research, training, outreach, community support, and sample storage activities. We currently use more than 7,000 gallons of liquid nitrogen per year, delivered in the form of ~120 separate 230-liter low-pressure rolling dewars. We receive and exchange these with a vendor each week, but the deliveries cannot be relied upon to arrive when scheduled, and the tanks routinely arrive empty or broken. The vendors also deliver high-pressure tanks that can damage our equipment. This requires AGGRC personnel to be present when the delivery truck arrives to ensure things are done properly and that the doors are locked when the delivery truck leaves. Delivery is especially not reliable after hurricanes or other disruptive events. The bottom line is that the center has grown to the extent that we require an external bulk storage (3,000-L) liquid nitrogen tank that can be installed on an existing concrete pad that was built for that purpose. Moreover, switching to bulk delivery will reduce per-liter nitrogen cost by 28%. In addition, our primary sample processing instrument (Minitube, Quattro) that fills, seals, and labels cryopreservation straws is >10 years old and is losing reliability. This piece of equipment is also used to provide labeling of cryopreservation straws used by the stock centers and other research community members. We need to replace this equipment to continue to provide reliable services. For example, the printer unit has been replaced in newer models and the supplier will no longer service the old version. Replacement would reduce use of the existing instrument and allow us to extend its working lifetime for back-up and overflow operation. Thus, we have great need to upgrade our liquid nitrogen supply and use, and to replace an essential piece of equipment that is used daily. Each of these improvements is in direct response to our growth as a service center addressing community needs, and our increased interaction as a Hub with all five NIH-funded national aquatic stock centers. If funded, this supplement will substantially improve operations relative to the parent grant, and it will provide a sustained powerful benefit to the overall research community. In addition, these improvements will not require additional funding beyond the supplement, and they will in fact substantially reduce our costs for liquid nitrogen. This work can be completed within the current project year (entering Year 4), and it does not overlap with work funded in the parent award or through previous supplemental funding.

项目摘要/摘要： 水产种质遗传资源中心(AGGRC)的活动已经扩大 在过去的5年里，我们正在为NIH资助的所有五个项目解决存储库开发问题 水产储备中心。可靠的液氮供应对大部分工作至关重要。巴吞鲁日 拥有强大的工业基础，液氮可以从当地供应商那里获得。然而，交付和 现场管理变得越来越麻烦，越来越成为我们研究的制约因素， 培训、外展、社区支持和样品储存活动。我们目前使用了7000多个 每年120加仑的液氮，以独立的230升低压轧制的形式提供 杜瓦尔。我们每周都会收到这些，并与供应商交换，但交货不可靠 一旦按计划到达，坦克通常是空的或坏的。供应商还提供 高压水箱会损坏我们的设备。这需要AGGRC人员在以下情况下在场 送货卡车到达时，确保事情做好，门也锁上了 送货卡车离开了。飓风或其他破坏性事件发生后，送货尤其不可靠。这个 底线是，中心已经发展到需要外部大容量存储的程度(3,000-L) 液氮储罐可以安装在为此目的而建造的现有混凝土垫板上。 此外，改用散装运输将使每升氮气成本降低28%。另外，我们的初选 填充、密封和标记低温保存吸管的样品处理仪(Minitube、Quattro)是 &gt；10岁，正在失去可靠性。这台设备还用于提供标签 库存中心和其他研究社区成员使用的超低温保存吸管。我们需要 更换此设备以继续提供可靠的服务。例如，打印机单元已被 更换为较新的型号，供应商将不再维修旧版本。替换将会是 减少对现有仪器的使用，并允许我们延长其备份和溢出的使用寿命 手术。因此，我们非常需要升级我们的液氮供应和使用，并更换一个 日常使用的必备设备。这些改进中的每一个都是对我们的 作为满足社区需求的服务中心增长，以及我们作为中心与所有 五个由美国国立卫生研究院资助的国家水产储备中心。如果获得资金，这一补充将大大改善 相对于母公司赠款的运营，它将为整体提供持续的强大好处 研究社区。此外，这些改进将不需要额外的资金 补充，它们实际上将大大降低我们的液氮成本。这项工作可以 在本项目年度(进入第4年)内完成，并不与在 家长奖励或通过以前的补充资金。