TOWARD GAINING INSIGHTS INTO THE MECHANISM OF SUBSTRATE TRANSPORT BY THE ASPART

深入了解 ASPAR 的基质传输机制

• 批准号: 8364334
• 负责人: Ivet Bahar
• 金额: $ 0.11万
• 依托单位: CARNEGIE-MELLON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-15 至 2013-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8364334
• 关键词: Aspartate; Automobile Driving; Binding; Biomedical Research; Cells; Funding; Glutamate Transporter; Grant; High Performance Computing; Homologous Gene; Human; Membrane Proteins; Molecular; Molecular Conformation; National Center for Research Resources; Neurotransmitters; Play; Principal Investigator; Process; Research; Research Infrastructure; Resources; Role; Running; Sodium; Source; Structure; United States National Institutes of Health; conformer; cost; extracellular; insight

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Neurotransmitter transporters are membrane proteins that utilize the electrochemical gradient of sodium for uphill translocation of their substrate/neurotransmitter from the extracellular region to the cell interior. This process involves a structural transition from outward-facing conformation to inward-facing conformation that enables the cyclical recognition/binding and release of the substrate. The crystal structure of the aspartate transporter GltPh, an archaeal homologue of human glutamate transporters, has been resolved in both conformations. We intend to perform MD runs using these two crystal structures towards gaining insights into molecular mechanisms of interactions that play a driving role in the passage between these two conformers.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 神经递质转运蛋白是一种膜蛋白，利用钠的电化学梯度将其底物/神经递质从细胞外区域上坡易位到细胞内部。该过程涉及从向外构象到向内构象的结构转变，从而实现底物的循环识别/结合和释放。 天冬氨酸转运蛋白 GltPh（人类谷氨酸转运蛋白的古菌同源物）的晶体结构已在两种构象中得到解析。我们打算使用这两种晶体结构进行 MD 运行，以深入了解在这两种构象异构体之间的传递中发挥驱动作用的相互作用的分子机制。