FOURIER DOMAIN LOW COHERENCE INTERFEROMETRIC MICROSCOPY

傅里叶域低相干干涉显微镜

• 批准号: 8364138
• 负责人: ZAHID YAQOOB
• 金额: $ 2.97万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8364138
• 关键词: Biological; Biomedical Research; Categories; Cell membrane; Funding; Glass; Goals; Grant; Image; Lasers; Lateral; Lighting; Location; Measurement; Microscope; Microscopy; National Center for Research Resources; Noise; Optics; Phase; Principal Investigator; Research; Research Infrastructure; Resolution; Resources; Sampling; Signal Transduction; Source; Specimen; Surface; System; Techniques; United States National Institutes of Health; cost; design; falls

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The goal of this project is to develop a low coherence reflection phase microscope with depth-resolved imaging capability for reflection-mode studies of cell membrane dynamics. Classical spectral domain phase microscope (SDPM) implementations employ common-path configuration in which the glass coverslip surface farther from the biological sample serves as a reference reflector. The common-path systems, however, compromise with the spatial resolution by using relatively low NA microscope objectives to simultaneously collect optical signal from the specimen as well as the reference surface. Moreover, the common-path systems use point illumination and fall into the category of single lateral point measurement techniques. To overcome the limitations of classical SDPM designs, we propose to build a phase-sensitive low coherence phase microscope with line-field illumination. The line-field illumination will not only allow self-phase referencing for common-mode noise rejection but also simultaneous depth-resolved phase measurement of multiple lateral locations.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其他NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 本计画的目标是发展一种具有深度分辨成像能力的低相干反射相位显微镜，以反射模式研究细胞膜的动力学。经典的谱域相位显微镜（SDPM）的实现采用公共路径配置，其中远离生物样品的玻璃盖玻片表面作为参考反射器。然而，公共路径系统，通过使用相对低NA的显微镜物镜，同时收集来自样品以及参考表面的光信号，与空间分辨率妥协。此外，共路径系统使用点照明并且属于单侧向点测量技术的类别。为了克服经典SDPM设计的局限性，我们提出建立一个相敏低相干相位显微镜与线场照明。线场照明不仅允许自相位参考以抑制共模噪声，而且还允许同时对多个横向位置进行深度分辨相位测量。