FOURIER DOMAIN LOW COHERENCE INTERFEROMETRIC MICROSCOPY

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们正在开发一种低相干傅立叶域相位显微镜（FDPM）的反射模式的细胞电运动的研究。经典的FDPM实现采用公共路径配置，其中远离生物样品的玻璃盖玻片表面用作参考反射器。然而，共同的路径FDPM系统，通过使用相对低NA的显微镜物镜，同时收集来自样品以及参考表面的光信号，与空间分辨率妥协。低NA显微镜物镜的使用还导致来自与生物样品相邻的玻璃盖片表面的相对较强的光学信号，因此导致来自样本的相位测量减小。此外，共光路FDPM系统使用点照明并且属于单侧向点测量技术的类别。 为了克服点照明共光路FDPM设置的局限性，我们提出了一种相位敏感的低相干相位显微镜与线场照明和一个单独的参考arm. The线场照明将不仅允许自相位参考共模噪声抑制，但也同时深度分辨多个横向位置的相位测量。如果需要，生物样品的二维相位成像将仅需要一维束扫描。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are developing a low coherence Fourier domain phase microscope (FDPM) for reflection-mode studies of cell electromotility. Classical FDPM implementations employ common-path configuration in which the glass coverslip surface farther from the biological sample serves as a reference reflector. The common-path FDPM systems, however, compromise with the spatial resolution by using relatively low NA microscope objectives to simultaneously collect optical signal from the specimen as well as the reference surface. The use of low NA microscope objectives also results in relatively stronger optical signal from glass coverslip surface adjacent to the biological sample, hence leading to diminished phase measurement from the specimen. Moreover, the common-path FDPM systems use point illumination and fall into the category of single lateral point measurement techniques. To overcome the limitations of point illumination common-path FDPM setups, we propose a phase-sensitive low coherence phase microscope with line-field illumination and a separate reference arm. The line-field illumination will not only allow self-phase referencing for common-mode noise rejection but also simultaneous depth-resolved phase measurement of multiple lateral locations. Only one-dimensional beam scanning will be required for two-dimensional phase imaging of biological sample, if desired.