FOURIER DOMAIN LOW COHERENCE INTERFEROMETRIC MICROSCOPY

傅里叶域低相干干涉显微镜

• 批准号: 8170388
• 负责人: ZAHID YAQOOB
• 金额: $ 2.85万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-01 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170388
• 关键词: Biological; Categories; Cell membrane; Computer Retrieval of Information on Scientific Projects Database; Funding; Glass; Grant; Image; Institution; Lateral; Lighting; Location; Measurement; Microscope; Microscopy; Noise; Optics; Phase; Research; Research Personnel; Resolution; Resources; Sampling; Scanning; Signal Transduction; Source; Specimen; Surface; System; Techniques; United States National Institutes of Health; arm; falls; two-dimensional

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are developing a low coherence Fourier domain phase microscope (FDPM) for reflection-mode studies of cell membrane dynamics. Classical FDPM implementations employ common-path configuration in which the glass coverslip surface farther from the biological sample serves as a reference reflector. The common-path FDPM systems, however, compromise with the spatial resolution by using relatively low NA microscope objectives to simultaneously collect optical signal from the specimen as well as the reference surface. The use of low NA microscope objectives also results in relatively stronger optical signal from glass coverslip surface adjacent to the biological sample, hence leading to diminished phase measurement from the specimen. Moreover, the common-path FDPM systems use point illumination and fall into the category of single lateral point measurement techniques. To overcome the limitations of point illumination common-path FDPM setups, we propose a phase-sensitive low coherence phase microscope with line-field illumination and a separate reference arm. The line-field illumination will not only allow self-phase referencing for common-mode noise rejection but also simultaneous depth-resolved phase measurement of multiple lateral locations. Only one-dimensional beam scanning will be required for two-dimensional phase imaging of biological sample, if desired.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们正在开发一种低相干傅里叶域位相显微镜(FDPM)，用于细胞膜动力学的反射模式研究。经典的FDPM实现采用公共路径配置，其中远离生物样品的玻璃覆盖滑动面作为参考反射器。然而，共路FDPM系统通过使用相对较低的NA显微镜物镜来同时从样品和参考表面采集光信号，从而损害了空间分辨率。使用低NA显微镜物镜也会导致来自邻近生物样品的玻璃盖片表面的相对较强的光信号，从而导致从样品测量的相位减少。此外，共路FDPM系统使用点照明，属于单侧向点测量技术的范畴。为了克服点照明共路FDPM装置的局限性，我们提出了一种具有线场照明和独立参照臂的相敏低相干位相显微镜。线场照明不仅可以为共模噪声抑制提供自相位参考，而且还可以同时进行多个横向位置的深度分辨相位测量。如果需要，生物样品的二维相位成像只需要一维束扫描。